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Senecavirus A nonstructural protein 3A Inhibits Ago2-mediated RNAi antiviral response
Mengru Luo1, Xingyi Xie1, Dengkun Wang1
1International Joint Research Center of National Animal Immunology, College of Veterinary Medicine, Henan Agricultural University, Zhengzhou, Henan, China; Ministry of Education Key Laboratory for Animal Pathogens and Biosafety, Henan Agricultural University, Zhengzhou, China.
Abstract:
Senecavirus A (SVA) can infect pigs of all ages and poses a significant risk to the swine industry. RNA interference (RNAi) is a conserved antiviral immune defense pathway in eukaryotes, and many viruses have been proven to encode viral proteins as viral RNAi suppressors (VSRs) to evade RNAi-mediated antiviral response. However, it is unclear whether SVA regulates RNAi for its infection and replication. In this study, it is found that SVA nonstructural protein 3 A has a VSR function. Mechanistically, 3 A effectively inhibited double-stranded RNA and small interfering RNA-induced RNAi, degrading Argonaute2 (Ago2) through autophagy. This paper demonstrated that SVA encoded 3 A as VSR to resist RNAi pathway, escaping host immunity and promoting viral replication, which expands the understanding of the interaction between SVA and its host.
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