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Related Concept Videos

Base Excision Repair01:54

Base Excision Repair

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One of the common DNA damages is the chemical alteration of single bases by alkylation, oxidation, or deamination. The altered bases cause mispairing and strand breakage during replication. This type of damage causes minimal change to the DNA double helix structure and can be repaired by the base excision repair (BER) pathways. BER corrects damaged DNA sequences by removing the damaged base and restoring the original base sequence using the complementary strand as a template.
The first step of...
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Long-patch Base Excision Repair01:02

Long-patch Base Excision Repair

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Since the discovery of the two BER pathways, there has been a debate about how a cell chooses one pathway over the other and the factors determining this selection. Numerous in vitro experiments have pointed out multiple determinants for the sub-pathway selection. These are:
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Base-pairing and DNA Repair02:27

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Nucleotide Excision Repair01:08

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Targeted Cancer Therapies02:57

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The targeted cancer therapies, also known as “molecular targeted therapies,” take advantage of the molecular and genetic differences between the cancer cells and the normal cells. It needs a thorough understanding of the cancer cells to develop drugs that can target specific molecular aspects that drive the growth, progression, and spread of cancer cells without affecting the growth and survival of other normal cells in the body.
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Conservative Site-specific Recombination and Phase Variation02:53

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Because the DNA segments are cut and reorganized in a direction-specific manner, site-specific recombination has emerged as an efficient genetic engineering technique. Flippase and Cyclization recombinases or Flp and Cre, respectively, are two members of the tyrosine recombinase family derived from bacteriophages, that are used to mediate site-specific DNA insertions, deletions, and targeted expression of proteins in mammalian cell lines.
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Related Experiment Video

Updated: May 20, 2025

Quantitative, Real-time Analysis of Base Excision Repair Activity in Cell Lysates Utilizing Lesion-specific Molecular Beacons
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Quantitative, Real-time Analysis of Base Excision Repair Activity in Cell Lysates Utilizing Lesion-specific Molecular Beacons

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Targeting base excision repair in precision oncology.

Nicola P Montaldo1, Hilde Loge Nilsen1, Diana L Bordin2

  • 1Department of Microbiology, Oslo University Hospital, Norway; Institute of Clinical Medicine, University of Oslo, Norway; CRESCO - Centre for embryology and healthy Development, University of Oslo, Norway.

DNA Repair
|May 13, 2025
PubMed
Summary

Targeting DNA repair pathways, like base excision repair (BER), offers a precision oncology strategy. This approach exploits tumor weaknesses to improve cancer treatment outcomes, especially in DNA damage response (DDR)-defective cancers.

Keywords:
APOBECBase Excision RepairCancerDNA glycosylasesPARPiTelomeres

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gDNA Enrichment by a Transposase-based Technology for NGS Analysis of the Whole Sequence of BRCA1, BRCA2, and 9 Genes Involved in DNA Damage Repair
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Area of Science:

  • Oncology
  • Molecular Biology
  • Cancer Therapeutics

Background:

  • Targeting the DNA damage response (DDR) is a crucial cancer therapy strategy.
  • Precision oncology aims to personalize treatments based on genetic vulnerabilities.
  • PARP inhibitors demonstrate the therapeutic potential of targeting DNA repair.

Purpose of the Study:

  • To explore the potential of targeting the base excision repair (BER) pathway in cancer therapy.
  • To highlight BER as a promising target, especially for DDR-defective tumors.
  • To review advancements in BER-targeted therapies for precision oncology.

Main Methods:

  • Literature review of DDR, BER, and precision oncology strategies.
  • Analysis of the role of BER in DNA repair mechanisms.
  • Discussion of clinical implications and future directions for BER-targeted therapies.

Main Results:

  • BER pathway targeting offers a novel approach to cancer treatment.
  • Tumors with deficiencies in other DNA repair pathways are particularly vulnerable to BER inhibition.
  • Advancements in biomarkers and drug development are paving the way for BER-targeted therapies.

Conclusions:

  • Targeting the BER pathway represents a significant advancement in precision oncology.
  • BER-targeted therapies hold promise for improving treatment outcomes in specific cancer types.
  • Further research and development in BER-targeted drugs are essential for clinical application.