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Related Concept Videos

Mouse Models of Cancer Study02:43

Mouse Models of Cancer Study

Mice have long served as models for studying human biology and pathology because of their phylogenetic and physiological similarity with humans. They are also easy to maintain and breed in the laboratory, and hence, many inbred strains are now available for research. Studies on mice have contributed immeasurably to our understanding of cancer biology.
The development of transgenic, knockout, and knock-in mice has led to an exponential increase in their use as model organisms in research,...
Mouse Models of Cancer Study02:43

Mouse Models of Cancer Study

Mice have long served as models for studying human biology and pathology because of their phylogenetic and physiological similarity with humans. They are also easy to maintain and breed in the laboratory, and hence, many inbred strains are now available for research. Studies on mice have contributed immeasurably to our understanding of cancer biology.
The development of transgenic, knockout, and knock-in mice has led to an exponential increase in their use as model organisms in research,...

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TAT-CRE inhalation enables tumor induction corresponding to adenoviral Cre-recombinase in a lung cancer mouse model.

Tabea Gewalt1, Anna M Dmitrieva2, Felix Elsner3

  • 1Department I of Internal Medicine, Faculty of Medicine and University Hospital Cologne, University of Cologne, Cologne, Germany.

Communications Biology
|May 13, 2025
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TAT-CRE offers a safer, S1 biosafety level alternative for inducing lung cancer in research models. This method, compared to S2 viral methods, shows similar tumor growth while presenting distinct micro-vessel and macrophage characteristics.

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Area of Science:

  • Oncology
  • Genetics
  • Molecular Biology

Background:

  • Cre-recombinase inducible systems are vital for cancer research, enabling gene manipulation and tumor induction.
  • Traditional methods include cross-breeding genetically engineered organisms and viral Cre-recombinase delivery.
  • Existing methods face challenges such as high animal numbers or restricted biosafety facility access.

Purpose of the Study:

  • To comprehensively compare TAT-CRE (biosafety level S1) and adenoviral Cre-recombinase (biosafety level S2) for inducing KrasG12D-driven lung adenocarcinomas with Trp53 depletion.
  • To elucidate similarities and differences in tumor characteristics induced by these two Cre-recombinase delivery methods.

Main Methods:

  • Induction of lung adenocarcinomas using TAT-CRE and adenoviral Cre-recombinase in genetically engineered mouse models.
  • In vivo tumor monitoring using computed tomography (CT).
  • Analysis of tumor characteristics via single-cell RNA sequencing, immunohistochemistry, and flow cytometry.

Main Results:

  • TAT-CRE induced lung tumors exhibited differences in micro-vessel density and macrophage populations compared to adenoviral-Cre induced tumors.
  • Both TAT-CRE and adenoviral Cre-recombinase methods resulted in comparable tumor onset and growth kinetics.
  • Despite micro-environmental differences, TAT-CRE proved effective for KrasG12D-driven lung adenocarcinoma induction.

Conclusions:

  • TAT-CRE represents a valuable, safer (S1 biosafety level) alternative for genetic engineering in cancer research.
  • This method facilitates autochthonous tumor induction and may be applicable beyond lung cancer models.
  • TAT-CRE overcomes the biosafety facility limitations associated with viral Cre-recombinase delivery.