Related Experiment Video
Updated: May 15, 2025

Author Spotlight: Unveiling the Potential of VSFG Microscopy in Studying Mesoscopically Heterogeneous Self-Assembled Structures
Published on: December 1, 2023
Advanced vibrational microscopes for life science
Ji-Xin Cheng1,2,3,4, Yuhao Yuan5, Hongli Ni5
1Department of Electrical and Computer Engineering, Boston University, Boston, MA, USA. jxcheng@bu.edu.
Abstract:
Providing molecular fingerprint information, vibrational spectroscopic imaging opens a new window to decipher the function of biomolecules in living systems. While classic vibrational microscopes based on spontaneous Raman scattering or mid-infrared absorption offer rich insights into sample composition, they have very small cross sections or poor spatial resolution. Nonlinear vibrational microscopy, based on coherent Raman scattering or optical photothermal detection of vibrational absorption, overcomes these barriers and enables high-speed and high-sensitivity imaging of chemical bonds in live cells and tissues. Here, we introduce various modalities, including their principles, strengths, weaknesses and data mining methods to the life sciences community. We further provide a guide for prospective users and an outlook on future technological advances.
Related Concept Videos
Overview of Microscopy Techniques
Atomic Force Microscopy
The AFM Probe
The probe is regarded as the heart of any AFM setup and comprises the...
Imaging Biological Samples with Optical Microscopy
In optical microscopy, the specimen to be viewed is placed on a glass slide and clipped on the stage...
Overview of Electron Microscopy
Confocal Fluorescence Microscopy
Super-resolution Fluorescence Microscopy

