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Oral Acid-Activated Hydrogen-Producing Nanoparticles Reduce Aortic Dissection Progression via RhoA/ROCK Inhibition in
Longwu Xu1, Lin Ma2, Xiufeng Xu3
1Department of Cardiology, Affiliated Hospital of Shandong Second Medical University, Weifang 261053, P.R. China.
ACS Applied Materials & Interfaces
|May 13, 2025
Summary
Hydrogen therapy using magnesium diboride nanosheets improved survival and reduced aortic dissection progression in mice. This treatment targets oxidative stress and the RhoA/ROCK pathway, offering a potential therapeutic strategy for this life-threatening condition.
Area of Science:
- Biomedical Engineering
- Cardiovascular Research
- Nanomedicine
Background:
- Aortic dissection (AD) is a critical vascular emergency with high mortality.
- Oxidative stress and cellular migration are key factors in AD development.
- Current therapeutic options for AD are limited.
Purpose of the Study:
- To investigate the therapeutic efficacy of hydrogen (H2) delivered by magnesium diboride nanosheets (MBNs) in a mouse model of AD.
- To elucidate the underlying molecular mechanisms of H2 therapy in AD.
- To assess the impact of H2 on survival and aortic integrity.
Main Methods:
- Induction of AD in mice using beta-aminopropionitrile.
- Treatment with H2-releasing MBNs.
- Assessment of survival rates, aortic histology, and false lumen formation.
- Transcriptomic analysis, Western blotting, immunohistochemistry, and immunofluorescence to evaluate the RhoA/ROCK pathway.
Main Results:
- H2 therapy via MBNs significantly improved survival rates in the AD mouse model.
- Treatment led to improved aortic wall structure and reduced false lumen formation.
- Transcriptomic and molecular analyses revealed significant downregulation of the RhoA/ROCK pathway and reduced oxidative stress markers.
Conclusions:
- Hydrogen delivered by MBNs demonstrates significant therapeutic potential for aortic dissection.
- The mechanism involves scavenging reactive oxygen species and inhibiting the RhoA/ROCK pathway.
- H2-based nanomedicine presents a promising avenue for treating AD.
