Butyrate Derivatives Exhibited Anti-Inflammatory Effects and Enhanced Intestinal Barrier Integrity in Porcine Cell...

Area of Science:

• Animal Science
• Immunology
• Gastroenterology

Background:

• Post-weaning diarrhea significantly impacts swine health and productivity.
• Dietary butyrate compounds offer a potential strategy to enhance disease resistance in pigs.

Purpose of the Study:

• To evaluate the effects of different butyrate derivatives on intestinal barrier integrity and inflammatory responses in porcine cells.
• To inform future in vivo studies on the application of butyrate supplements for pigs.

Main Methods:

• Porcine intestinal epithelial cells (IPEC-J2) were used to assess cell viability and intestinal barrier integrity via MTT assay and transepithelial electrical resistance (TEER).
• Porcine alveolar macrophages (PAMs) were cultured and treated with butyrate derivatives, with or without lipopolysaccharide (LPS) challenge, to measure inflammatory cytokine production (TNF-α, IL-1β) using ELISA.

Main Results:

• Butyric acid, sodium butyrate, and monobutyrin significantly increased TEER in IPEC-J2 cells, indicating improved intestinal barrier function.
• Butyric acid and sodium butyrate treatments significantly reduced TNF-α production in LPS-challenged PAMs.
• Different butyrate derivatives demonstrated anti-inflammatory properties and enhanced intestinal barrier integrity in vitro.

Conclusions:

• Butyrate derivatives show promise as dietary supplements to improve swine health.
• These findings support the use of butyrate-based compounds to enhance disease resistance and gut health in pigs.