The Influence of Different Preservation Protocols on the Teeth's Osteoinductive Characteristics: An In Vitro Study.
Filiberto Mastrangelo1, Sara Franceschelli2, Ciro Annicchiarico3
1Department of Clinical and Experimental Medicine, University of Foggia, Via Rovelli n. 60, 77100 Foggia, Italy.
International Journal of Molecular Sciences
|May 14, 2025
Summary
Freshly extracted teeth preserve the most osteoinductive proteins for bone regeneration. Storing teeth at room temperature significantly reduces bone morphogenetic protein-2 (BMP-2) and collagen type-I (COL-I) levels.
Area of Science:
- Biomaterials Science
- Regenerative Medicine
- Oral Surgery
Background:
- Tooth preservation methods can impact the osteoinductive potential of extracted teeth.
- Understanding the effects of storage on key bone regeneration proteins is crucial for clinical applications.
Purpose of the Study:
- To evaluate in vitro how different tooth preservation methods affect the osteoinductive characteristics of extracted teeth.
- To determine the optimal storage conditions for maximizing osteoinductive protein content in teeth treated with the Tooth Transformer (TT) device.
Main Methods:
- Forty extracted teeth were divided into four groups: room-temperature (RT), frozen, RIPA, and fresh samples.
- Teeth were processed using the Tooth Transformer (TT) device for mincing, demineralization, and disinfection.
- Enzyme-Linked ImmunoSorbent Assay (ELISA) and Western Blot analyses were performed to quantify osteoinductive proteins.
Main Results:
- Fresh teeth retained the highest levels of bone morphogenetic protein-2 (BMP-2) and collagen type-I (COL-I).
- Room-temperature storage led to significant loss of BMP-2 and COL-I.
- Fresh and frozen samples showed higher levels of Mineralization Protein LIM-1 (LMP-1) compared to other groups.
Conclusions:
- Prompt treatment of extracted teeth with the TT device is recommended to maximize osteoinductive potential.
- Freshly preserved teeth offer the greatest potential for bone preservation and regeneration procedures.
- Storage conditions significantly influence the availability of key osteoinductive factors in extracted teeth.
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