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Nitrate Modulates Fruit Lignification by Regulating CgLAC3 Expression in Pomelo.
Changhong Lai1, Huiwen Zhou1, Hong Liao1
1Root Biology Center, Fujian Agriculture and Forestry University, Fuzhou 350002, China.
International Journal of Molecular Sciences
|May 14, 2025
Summary
Nitrate levels significantly increase lignin in pomelo fruit, impacting quality. Managing nitrate-nitrogen fertilization can help improve pomelo fruit lignification and quality.
Area of Science:
- Plant Science
- Agricultural Science
- Biochemistry
Background:
- Fruit lignification negatively impacts pomelo quality and economic value.
- Understanding the regulation of fruit lignification is crucial for improving crop yields.
Purpose of the Study:
- To investigate the relationship between nutrient status and lignification in pomelo juice sacs.
- To identify key enzymes and regulatory pathways involved in nitrate-modulated lignification.
Main Methods:
- Correlation analysis between nitrate concentration and lignin concentration in pomelo juice sacs.
- Machine learning-based regression models to assess nitrate's impact on lignin accumulation.
- Field trials in pomelo orchards and experiments with pear and soybean.
- Transcriptomic analysis to identify differentially expressed genes, focusing on laccases.
- Gene overexpression studies in pomelo and soybean to validate enzyme function.
Main Results:
- A significant positive correlation was found between nitrate (NO3-) concentration and lignin concentration in pomelo.
- Nitrate was identified as a key factor explaining over 60% of lignin accumulation.
- Pomelo laccase genes (CgLACs), particularly CgLAC3, were significantly upregulated by nitrate.
- Overexpression of CgLAC3 led to increased lignin content in pomelo and soybean tissues.
Conclusions:
- Nitrate modulates pomelo fruit lignification primarily through the regulation of CgLAC3 expression.
- Nitrate-nitrogen fertilization strategies can be adjusted to manage fruit lignification and enhance pomelo quality.
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