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Role of c-ABL in DENV-2 Infection and Actin Remodeling in Vero Cells
Grace Paola Carreño-Flórez1, Alexandra Milena Cuartas-López1, Ryan L Boudreau2
1Translational Medicine Group-School of Medicine, Universidad de Antioquia, Medellín 050010, Colombia.
Abstract:
In this study, we address the role of c-ABL (cellular Abelson Tyr kinase) in the cytoskeletal rearrangements induced by DENV (Dengue virus) infection in mammalian cells. Using the specific inhibitor imatinib and targeted RNA interference, we show that c-ABL is necessary for viral entry and subsequent ENV (DENV envelope protein) accumulation in infected cells. In addition, c-ABL targeting attenuates F-actin reorganization induced by DENV infection. Together with the involvement of c-ABL in endothelial dysfunction induced by DENV and host secreted factors, our findings strongly suggest that c-ABL is a potential host-targeted antiviral that could control DENV infection and/or its evolution to more severe forms of the disease.
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