Related Experiment Video

Updated: May 17, 2025

Characterization of Cell Membrane Extensions and Studying Their Roles in Cancer Cell Adhesion Dynamics
08:11

Characterization of Cell Membrane Extensions and Studying Their Roles in Cancer Cell Adhesion Dynamics

Published on: March 26, 2018

7.5K

Ankrd1 Promotes Lamellipodia Formation and Cell Motility via Interaction with Talin-1 in Clear Cell Renal Cell

Yuki Takai1, Sei Naito1, Hiromi Ito1

  • 1Department of Urology, Faculty of Medicine, Yamagata University, 2-2-2 Iida-nishi, Yamagata 990-9585, Japan.

International Journal of Molecular Sciences
|May 14, 2025
PubMed

Insights

Ankyrin repeat domain 1 (Ankrd1) promotes clear cell renal cell carcinoma (ccRCC) cell motility and lamellipodia formation. High Ankrd1 expression correlates with poor ccRCC prognosis, suggesting its role in metastasis.

Area of Science:

  • Oncology
  • Molecular Biology
  • Cell Biology

Background:

  • Ankyrin repeat domain 1 (Ankrd1) is linked to cardiomyopathy but its role in clear cell renal cell carcinoma (ccRCC) is unclear.
  • Yes-associated protein (YAP) regulates Ankrd1, which is implicated in various cellular processes.

Purpose of the Study:

  • To investigate the expression, regulation, and functional role of Ankrd1 in ccRCC.
  • To determine Ankrd1's impact on ccRCC cell motility and its association with patient prognosis.

Main Methods:

  • Analysis of The Cancer Genome Atlas (TCGA) cohort for Ankrd1 expression and prognosis.
  • Examination of Ankrd1 regulation by YAP and ERK5 in ccRCC cell lines.
  • Functional studies involving Ankrd1 silencing and overexpression in ccRCC cells.
  • Immunohistochemical analysis of Ankrd1 expression in primary and metastatic RCC tissues.

Main Results:

  • High Ankrd1 expression is associated with poor prognosis in ccRCC patients.
  • Ankrd1 expression is regulated by YAP and ERK5 in ccRCC.
  • Ankrd1 silencing decreases ccRCC cell motility and lamellipodia formation.
  • Ankrd1 overexpression enhances ccRCC cell motility.
  • Ankrd1 colocalizes with F-actin and talin-1 in lamellipodia, repressing integrin pathway activation.
  • Ankrd1 is highly expressed in metastatic RCC tissues compared to primary tissues.

Conclusions:

  • Ankrd1 plays a critical role in ccRCC cell motility via lamellipodia formation.
  • Ankrd1 is a potential prognostic biomarker and therapeutic target for ccRCC, particularly in metastatic disease.
Keywords:
lamellipodiametastasisrenal cell carcinoma

More Related Videos

Impedance-based Real-time Measurement of Cancer Cell Migration and Invasion09:23

Impedance-based Real-time Measurement of Cancer Cell Migration and Invasion

Published on: April 2, 2020

6.4K
Measuring Cell-Edge Protrusion Dynamics during Spreading using Live-Cell Microscopy05:50

Measuring Cell-Edge Protrusion Dynamics during Spreading using Live-Cell Microscopy

Published on: November 1, 2021

2.3K

Related Concept Videos

Intracellular Signaling Affects Focal Adhesions01:17

Intracellular Signaling Affects Focal Adhesions

Integrins act both as extracellular input receivers and as intracellular processing activators. As their name suggests, integrins are entirely integrated into the membrane structure. Their hydrophobic membrane-spanning regions interact with the phospholipid bilayer's hydrophobic region. These membrane receptors provide extracellular attachment sites for effectors like hormones and growth factors. They activate intracellular response cascades when their effectors are bound and active.
Some...
2.5K
Cancer Cell Migration through Invadopodia01:35

Cancer Cell Migration through Invadopodia

Invadosome is a broad category of cell surface structures with proteolytic activity that  degrades the extracellular matrix (ECM). Invadosomes are present in normal cell types, including macrophages, endothelial cells, and neurons, as well as tumor cells. Although the macrophage podosomes and tumor cell invadopodia are classified as invadosomes, they have different structures, molecular pathways, and functions. Podosomes are short structures that last for a few minutes. However,...
2.3K
Mechanism of Lamellipodia Formation01:31

Mechanism of Lamellipodia Formation

Cells migrating in response to external stimuli form lamellipodia, which are thin membrane protrusions supported by a mesh of linked, branched, or unbranched actin filaments. These actin filaments interact with myosin motor proteins, creating the dynamic actomyosin complex within the cytoskeleton. Contractility, or the ability to generate contractile stress, is inherent to the actomyosin complex. It helps cells detect the stiffness of the surrounding ECM and exert contractile force for...
2.5K
Cell Motility through Blebbing01:16

Cell Motility through Blebbing

Blebs are a type of membrane protrusion formed by the internal hydrostatic pressure of the cytoplasm. Blebs are observed in several cell types, including fibroblasts, immune cells, and single-celled organisms like the amoeba. The primary function of blebs is cell locomotion and apoptosis, but they are also found during necrosis and cell division. The life cycle of a bleb comprises an initiation phase followed by the expansion and retraction phases.
Blebbing Through the Matrix
In multicellular...
1.8K
Cytoskeletal Coordination in Cell Migration01:32

Cytoskeletal Coordination in Cell Migration

A migrating cell changes its shape during the cyclic events of attachment and detachment from the substratum and repositions the cell organelles correspondingly. These complex events are orchestrated by the dynamic cytoskeletal network comprising actin filaments, intermediate filaments, and microtubules. Cytoskeletal crosstalk — the direct and indirect communication between the different components — is crucial for this coordination. Direct communication involves various linker...
4.7K
Cadherins in Tissue Organization01:19

Cadherins in Tissue Organization

The cadherins are a superfamily of cell adhesion molecules comprising over 180 variants, with specific tissues expressing a particular combination of cadherin types. Cadherins generally exhibit homophilic binding; i.e., cadherins on one cell bind to cadherins of the same or closely related type on another cell. Thus, cells of the same type have a specific affinity to bind to each other and sort themselves into clusters to form tissues.
Cell Sorting During Development
Cell sorting plays an...
2.9K