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Development of Amelogenin-chitosan Hydrogel for In Vitro Enamel Regrowth with a Dense Interface

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Innovative Amber-Based Composite-From Mechanochemical Synthesis and Physicochemical Characterization to Application

Małgorzata Wiśniewska¹, Victoria Paientko², Iwona Ostolska¹

¹Department of Radiochemistry and Environmental Chemistry, Institute of Chemical Sciences, Faculty of Chemistry, Maria Curie-Sklodowska University in Lublin, M. Curie-Sklodowska Sq. 3, 20-031 Lublin, Poland.

International Journal of Molecular Sciences

|May 14, 2025

Summary

A novel composite of amber, diatomite, and Phytokeratin™ (hydrolyzed plant protein) was created using mechanochemistry. This sustainable ingredient enhances cosmetic formulations by maximizing active substance release.

Keywords:

active substances release amber-based composite composition optimization cosmetic application diatomite carrier molecular modeling succinic acid

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Area of Science:

Materials Science
Cosmetic Science
Sustainable Chemistry

Background:

Growing demand for sustainable development in cosmetics.
Need to maximize natural ingredient utilization while minimizing environmental impact.
Focus on eco-friendly cosmetic formulations.

Purpose of the Study:

To develop a three-component natural composite using mechanochemical synthesis.
To maximize the release of succinic acid and Phytokeratin™ in aqueous solution.
To evaluate the composite's suitability for cosmetic applications.

Main Methods:

Mechanochemical synthesis of amber, diatomite, and Phytokeratin™.
Characterization using SEM, TG/DTG, FTIR, and UV-Vis spectrophotometry.
Quantum chemical calculations using Density Functional Theory (DFT).

Main Results:

Optimized composite showed favorable release characteristics and structural properties.
Physicochemical properties were thoroughly analyzed.
DFT confirmed molecular interactions, stability, and functional integration.

Conclusions:

The developed natural composite is suitable for cosmetic applications.
The composite was successfully incorporated into eco-friendly products like solid shampoo and nail conditioner.
This research contributes to sustainable practices in the cosmetic industry.