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A Novel CFEM Effector in Fusarium verticillioides Required for Virulence Involved in Plant Immunity Suppression and

Huan Li1, Shumila Ishfaq1, Xiaoyan Liang1

  • 1Institute of Food Science and Technology, Chinese Academy of Agricultural Sciences/Key Laboratory of Agro-Products Quality and Safety Control in Storage and Transport Process, Ministry of Agriculture and Rural Affairs, Beijing 100193, China.

International Journal of Molecular Sciences
|May 14, 2025
PubMed

Insights

Fusarium verticillioides uses the FvCFEM12 effector to suppress plant immunity and maintain its cell wall integrity. This dual function is crucial for fungal pathogenicity and host-pathogen interactions.

Area of Science:

  • Plant Pathology
  • Mycology
  • Molecular Plant-Microbe Interactions

Background:

  • Common in Fungal Extracellular Membrane (CFEM) effectors are vital for fungal-specific interactions with hosts.
  • CFEM proteins are well-studied in plant pathogens, but their role in Fusarium verticillioides was unknown.

Purpose of the Study:

  • To identify and characterize CFEM proteins in Fusarium verticillioides.
  • To elucidate the function of FvCFEM12 in host-pathogen interactions and fungal biology.

Main Methods:

  • Systematic identification of CFEM proteins in F. verticillioides.
  • Functional analysis using heterologous expression, gene deletion, and interaction studies in plant models (Nicotiana benthamiana, maize).

Main Results:

  • 19 CFEM proteins were identified in F. verticillioides, with FvCFEM12 showing secretion and induction during infection.
  • FvCFEM12 suppressed plant cell death, compromised plant immunity, and its deletion impaired fungal virulence and cell wall integrity.
  • FvCFEM12 interacts with maize receptor kinase ZmWAK17, and ZmWAK17 silencing increases susceptibility to F. verticillioides.

Conclusions:

  • FvCFEM12 is a dual-function effector suppressing plant immunity and maintaining fungal cell wall integrity.
  • FvCFEM12 plays a significant role in orchestrating Fusarium verticillioides pathogenicity at the host-pathogen interface.
Keywords:
CFEM domainFusarium verticillioideseffectorshost–pathogen interactionmaizepathogenicity

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