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Non-Thermal Plasma Attenuates TNF-α-Induced Endothelial Inflammation via ROS Modulation and NF-κB Inhibition.

Joo-Hak Kim1, Seonhee Kim2, Shuyu Piao2

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Non-thermal plasma (NTP) reduces inflammation in blood vessel cells by lowering reactive oxygen species and suppressing key inflammatory signals. This suggests NTP could be a new treatment for vascular inflammation.

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NF-κBendothelial cell inflammationnon-thermal plasmareactive oxygen species

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Area of Science:

  • Biomedical Engineering
  • Cell Biology
  • Immunology

Background:

  • Non-thermal plasma (NTP) shows therapeutic potential due to anti-inflammatory effects.
  • Molecular mechanisms of NTP on vascular endothelial inflammation require further elucidation.

Purpose of the Study:

  • To investigate the effects of NTP on tumor necrosis factor-alpha (TNF-α)-induced inflammation in human umbilical vein endothelial cells (HUVECs).
  • To explore the underlying molecular mechanisms of NTP's anti-inflammatory action in endothelial cells.

Main Methods:

  • HUVECs were treated with NTP following TNF-α stimulation.
  • Assessed intracellular reactive oxygen species (ROS) levels.
  • Measured mRNA expression of pro-inflammatory cytokines (TNF-α, IL-1β, IL-6) and adhesion molecules (ICAM-1, VCAM-1).
  • Analyzed NF-κB signaling pathway activation via p65 nuclear translocation.

Main Results:

  • NTP treatment significantly reduced intracellular ROS levels in HUVECs.
  • NTP downregulated the expression of ICAM-1 and VCAM-1, key markers of endothelial activation.
  • NTP suppressed the mRNA expression of pro-inflammatory cytokines TNF-α, IL-1β, and IL-6.
  • NTP inhibited the nuclear translocation of phosphorylated NF-κB p65, indicating suppression of NF-κB signaling.

Conclusions:

  • NTP effectively modulates inflammatory responses in endothelial cells.
  • NTP attenuates ROS generation and suppresses NF-κB-mediated signaling pathways.
  • NTP presents a potential therapeutic strategy for vascular inflammation and related diseases.