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Related Concept Videos

CRISPR01:59

CRISPR

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Genome editing technologies allow scientists to modify an organism’s DNA via the addition, removal, or rearrangement of genetic material at specific genomic locations. These types of techniques could potentially be used to cure genetic disorders such as hemophilia and sickle cell anemia. One popular and widely used DNA-editing research tool that could lead to safe and effective cures for genetic disorders is the CRISPR-Cas9 system. CRISPR-Cas9 stands for Clustered Regularly Interspaced...
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Because the DNA segments are cut and reorganized in a direction-specific manner, site-specific recombination has emerged as an efficient genetic engineering technique. Flippase and Cyclization recombinases or Flp and Cre, respectively, are two members of the tyrosine recombinase family derived from bacteriophages, that are used to mediate site-specific DNA insertions, deletions, and targeted expression of proteins in mammalian cell lines.
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CRISPR and crRNAs02:53

CRISPR and crRNAs

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Bacteria and archaea are susceptible to viral infections just like eukaryotes; therefore, they have developed a unique adaptive immune system to protect themselves. Clustered regularly interspaced short palindromic repeats and CRISPR-associated proteins (CRISPR-Cas) are present in more than 45% of known bacteria and 90% of known archaea.
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Homologous Recombination02:31

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The basic reaction of homologous recombination (HR) involves two chromatids that contain DNA sequences sharing a significant stretch of identity. One of these sequences uses a strand from another as a template to synthesize DNA in an enzyme-catalyzed reaction. The final product is a novel amalgamation of the two substrates. To ensure an accurate recombination of sequences, HR is restricted to the S and G2 phases of the cell cycle. At these stages, the DNA has been replicated already and the...
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Updated: May 15, 2025

A New Toolkit for Evaluating Gene Functions using Conditional Cas9 Stabilization
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A New Toolkit for Evaluating Gene Functions using Conditional Cas9 Stabilization

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Conditional Control of CRISPR/Cas9 Function by Chemically Modified Oligonucleotides.

Liangliang Wang1,2, Yan Liu2, Hongjun Song2

  • 1School of Biological and Pharmaceutical Engineering, Lanzhou Jiaotong University, Lanzhou 730070, China.

Molecules (Basel, Switzerland)
|May 14, 2025
PubMed
Summary
This summary is machine-generated.

Chemically modified guide RNAs offer precise control over CRISPR gene editing. These innovations enhance precision and efficiency, paving the way for safer gene therapies and advanced functional genomics research.

Keywords:
CRISPR/Cas9chemical modificationconditional controlgene editingguide RNA (gRNA)

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Area of Science:

  • Molecular Biology
  • Biotechnology
  • Chemical Biology

Background:

  • CRISPR-Cas9 gene editing holds significant promise for therapeutic applications.
  • Clinical translation is hindered by challenges in achieving precise spatiotemporal control and mitigating off-target effects.

Purpose of the Study:

  • To review strategies for chemically modifying guide RNAs (gRNAs) to improve CRISPR-Cas9 editing.
  • To explore methods for achieving precise spatiotemporal and dose-dependent regulation of gene editing.

Main Methods:

  • Introduction of conditional responsive elements into oligonucleotides.
  • Utilizing photosensitive groups, small-molecule responsive units, and supramolecular structures for gRNA regulation.

Main Results:

  • Demonstrated precise spatiotemporal and dose-dependent control of CRISPR/Cas9 function through chemical modifications.
  • Enhanced precision, efficiency, and controllability of gene editing processes.

Conclusions:

  • Chemical modifications of gRNAs represent a powerful approach to overcome limitations in CRISPR/Cas9 technology.
  • Future directions involve further advancements in chemical regulation for broader CRISPR applications.