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DNA probes are fragments of DNA labeled with a reporter tag to enable their detection or purification. The resulting labeled DNA probes can then hybridize to target nucleic acid sequences through complementary base-pairing, and may be used to recover or identify these regions.
Radioisotopes, fluorophores, or small molecule binding partners like biotin or digoxigenin, are the most widely used reporter tags for labeling DNA probes. These labels can be attached to the probe DNA molecule via...
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Related Experiment Video

Updated: May 15, 2025

Author Spotlight: An Efficient Methodology to Confidently Differentiate and Characterize Fentanyl Analogs
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An "On-Off" AIE-Based Lock-and-Key Fluorescent Probe System for Detection of Fentanyl/Norfentanyl.

Jing Sun1,2, Junge Zhi3, Li Zhang4

  • 1National Key Laboratory of Veterinary Public Health Security, College of Veterinary Medicine, China Agricultural University, Beijing 100193, China.

Molecules (Basel, Switzerland)
|May 14, 2025
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Summary

A new fluorescent probe, TP-CF3-COOH, enables rapid and sensitive detection of fentanyl and its metabolite norfentanyl. This probe

Keywords:
aggregation-induced luminescence probefentanylself-assembly drivespatial specific recognitionthrough-space charge transfer (TSCT)

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Area of Science:

  • * Analytical Chemistry
  • * Materials Science
  • * Forensic Science

Background:

  • * Fentanyl misuse presents significant societal risks.
  • * Accurate and swift field detection of fentanyl is challenging.
  • * Development of sensitive detection methods is crucial.

Purpose of the Study:

  • * To design and synthesize a novel fluorescent probe for fentanyl detection.
  • * To establish a fluorescence "on-off" mechanism for fentanyl and norfentanyl recognition.
  • * To enable rapid, cost-effective, and sensitive onsite screening.

Main Methods:

  • * Synthesis of a tetraphenyl butadiene-based fluorescent probe (TP-CF3-COOH).
  • * Investigation of the probe's interaction with fentanyl and norfentanyl.
  • * Utilization of fluorescence spectroscopy and theoretical calculations.
  • * Establishment of a quantitative detection assay.

Main Results:

  • * TP-CF3-COOH probe exhibits a fluorescence "on-off" mechanism upon binding fentanyl/norfentanyl.
  • * Fentanyl/norfentanyl interaction induces spontaneous self-assembly and fluorescence quenching.
  • * Theoretical calculations confirm intermolecular charge transfer and blue shift in emission.
  • * Quantitative detection achieved in the range of 1 × 10⁻²–1 × 10³ μg/L.
  • * Limits of detection are as low as 2 × 10⁻⁴ μg/L (fentanyl) and 3 × 10⁻⁴ μg/L (norfentanyl).

Conclusions:

  • * The TP-CF3-COOH probe offers a cost-effective, rapid, and sensitive method for fentanyl detection.
  • * The probe's self-assembly mechanism provides a unique recognition platform.
  • * This technology holds significant potential for onsite screening of fentanyl and its analogues.