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Updated: May 15, 2025

Isolation and Functional Assessment of Human Breast Cancer Stem Cells from Cell and Tissue Samples
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Published on: October 2, 2020

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Effects of Integrin-Linked Kinase Silencing Combined With Trichostatin A on Cancer Stem Cells

Yulu Wu1,2, Zhiyong Guo1,2, Zhangao Li1

  • 1State Key Laboratory of Oral Diseases & National Center for Stomatology & National Clinical Research Center for Oral Diseases, West China Hospital of Stomatology, Sichuan University, Chengdu, Sichuan, China.

Oral Diseases
|May 14, 2025
PubMed
Abstract

Insights

Targeting cancer stem cells (CSCs) in oral squamous cell carcinoma (OSCC) using integrin-linked kinase (ILK) silencing and trichostatin A (TSA) treatment effectively reduced tumor growth and metastasis. This approach shows promise for CSC-directed therapies in OSCC.

Area of Science:

  • Oncology
  • Cancer Stem Cell Biology

Background:

  • Oral squamous cell carcinoma (OSCC) exhibits high invasiveness and metastasis.
  • Cancer stem cells (CSCs) are key drivers of OSCC progression and therapeutic resistance.

Purpose of the Study:

  • To investigate the therapeutic potential of integrin-linked kinase (ILK) silencing and trichostatin A (TSA) treatment on OSCC CSCs.
  • To assess the impact of these interventions on CSC properties and OSCC progression.

Main Methods:

  • CSCs were isolated from OSCC samples and cell lines using side population (SP) analysis.
  • Assays included MTT, wound healing, invasion, cell cycle, and apoptosis analyses.
  • In vivo experiments evaluated tumor growth inhibition.

Main Results:

  • Elevated SP cell content correlated with advanced pathological classification, metastasis, and recurrence.
  • ILK silencing and TSA treatment reduced CSC proliferation, migration, and invasion, while increasing apoptosis.
  • Combined treatment significantly inhibited tumor growth in vivo.

Conclusions:

  • Targeting OSCC CSCs with ILK silencing and TSA is an effective strategy.
  • This approach offers a promising avenue for CSC-directed therapies in OSCC management.
Keywords:
CSCILKOSCCTSAcell isolationoral oncologyside population

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