Single-cell views of fate reprogramming in de novo organogenesis

De novo organogenesis is a key process in plant development and regeneration, enabling plants to adapt and survive under suboptimal conditions. Studying the molecular mechanisms of cellular reprogramming that drives new organ formation has been challenging since only a subset of cells among heterogeneous cell populations change the cell fate. Recent advancements in single-cell technologies, however, have begun to provide unprecedented insights into the cell identities and their developmental trajectories, offering a deeper understanding of cell fate transitions during this process. In this review we highlight how single-cell approaches help uncover the regulatory networks that govern cell fate reprogramming and propose future directions for improving temporal and spatial resolution to further advance this emerging field.