The undescribed alkaloids from Jacobaea cannabifolia (less.) E. Wiebe and their anti-inflammatory activities

From the whole herb of Jacobaea cannabifolia (Less.) E. Wiebe, one new alkaloid named cannabliold E (1), one new natural alkaloid derivative named methyl 4-(2-oxo-1-pyrrolidinyl) benzene acetate (2) along with seven known alkaloids (3-9) were successfully isolated. The structures of the newly identified compounds were elucidated using NMR spectroscopy and HR-ESI-MS. The absolute configurations of compound 1 were assigned through electronic circular dichroism (ECD) analysis. Identification of the known compounds was achieved by comparing their 1D NMR data with literature references. All isolated compounds were assessed for anti-inflammatory activity against LPS-induced RAW 264.7 cells; results indicated that compounds 6 and 8 exhibited significant anti-inflammatory effects with IC₅₀ values below 1.59 ± 0.18 μM.