Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

EPS and iPS Cells in Disease Research01:21

EPS and iPS Cells in Disease Research

Embryonic and induced pluripotent stem cells are excellent models for disease research because of their ability to self-renew and differentiate into most cell types. Somatic cells from a patient are isolated and reprogrammed into induced pluripotent stem cells or iPSCs. These iPSCs are later differentiated into the desired cell type, which mirrors the diseased cell of the patient. In this way, disease models have been created for investigating diseases such as Down syndrome, type I diabetes,...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

A Bayesian Learning Model for Joint Risk Prediction of Alcohol and Cannabis Use Disorders.

Statistics in medicine·2026
Same author

Combined pharmacotherapy and psychotherapy impact on opioid use disorder treatment.

The American journal of managed care·2026
Same author

CBCRisk-Mastectomy: A Prediction Tool to Aid Contralateral Prophylactic Mastectomy Decision- making.

The Journal of surgical research·2026
Same author

Engineering copper nanoparticle/polysaccharide-immobilized cotton gauze for accelerated healing of <i>Staphylococcus aureus</i>-infected dermal wounds.

Journal of materials chemistry. B·2026
Same author

MRGPRX2 as a novel therapeutic target in headache.

Neurotherapeutics : the journal of the American Society for Experimental NeuroTherapeutics·2026
Same author

Multicenter Clinical Comparison of the 10-Minute AMDI Fast PCR Mini Respiratory Panel and the Cepheid Xpert Xpress CoV-2/Flu/RSV plus.

The Journal of molecular diagnostics : JMD·2026

Related Experiment Video

Updated: Jul 12, 2026

Recording Network Activity in Spinal Nociceptive Circuits Using Microelectrode Arrays
11:28

Recording Network Activity in Spinal Nociceptive Circuits Using Microelectrode Arrays

Published on: February 9, 2022

2.8K

Profiling human iPSC-derived sensory neurons for analgesic drug screening using a multi-electrode array.

Christian Kuete Fofie1, Rafael Granja-Vazquez1, Vincent Truong2

  • 1Department of Neuroscience and Center for Advanced Pain Studies, University of Texas at Dallas, Richardson, TX 75080, USA.

Cell Reports Methods
|May 14, 2025
PubMed
Summary

Researchers developed a novel high-content screening (HCS) platform using human induced pluripotent stem cell-derived nociceptors to discover new pain relief medications. This innovative approach aims to improve the translation of preclinical findings to human treatments for chronic pain.

Keywords:
CP: Stem cellDRGanalgesic discoverychronic painhiPSChigh-content screeninghuman induced pluripotent stem cellnociceptor

More Related Videos

Open-Source Real-Time Closed-Loop Electrical Threshold Tracking for Translational Pain Research
10:28

Open-Source Real-Time Closed-Loop Electrical Threshold Tracking for Translational Pain Research

Published on: April 21, 2023

1.2K
Author Spotlight: In Vitro and In Vivo Models to Enhance Chronic Pain Treatment Efficacy
06:56

Author Spotlight: In Vitro and In Vivo Models to Enhance Chronic Pain Treatment Efficacy

Published on: June 28, 2024

888

Related Experiment Videos

Last Updated: Jul 12, 2026

Recording Network Activity in Spinal Nociceptive Circuits Using Microelectrode Arrays
11:28

Recording Network Activity in Spinal Nociceptive Circuits Using Microelectrode Arrays

Published on: February 9, 2022

2.8K
Open-Source Real-Time Closed-Loop Electrical Threshold Tracking for Translational Pain Research
10:28

Open-Source Real-Time Closed-Loop Electrical Threshold Tracking for Translational Pain Research

Published on: April 21, 2023

1.2K
Author Spotlight: In Vitro and In Vivo Models to Enhance Chronic Pain Treatment Efficacy
06:56

Author Spotlight: In Vitro and In Vivo Models to Enhance Chronic Pain Treatment Efficacy

Published on: June 28, 2024

888

Area of Science:

  • Neuroscience
  • Pharmacology
  • Biotechnology

Background:

  • Chronic pain affects millions globally, with limited effective treatments due to challenges in translating preclinical research to human applications.
  • Current analgesic discovery pipelines face significant hurdles in predicting human efficacy from animal models.

Purpose of the Study:

  • To develop and validate a high-content screening (HCS) platform for identifying novel analgesics targeting the peripheral nervous system.
  • To utilize human induced pluripotent stem cell (hiPSC)-derived nociceptors for improved preclinical-to-human translation in drug discovery.

Main Methods:

  • Cultured hiPSC-derived nociceptors on multi-well microelectrode arrays, achieving stable and high neuronal activity.
  • Assessed drug effects on neuronal activity using robust assay performance (Z' > 0.5) after 28 days of culture.
  • Performed pharmacological profiling against known analgesic targets and conducted transcriptomic analysis.

Main Results:

  • The platform demonstrated stable neuronal activity and robust assay performance for drug screening.
  • Pharmacological tests confirmed the platform's ability to detect responses to various analgesic targets, including ion channels and receptors.
  • Screening of over 700 natural compounds revealed the platform's potential for discovering uncharacterized analgesics.

Conclusions:

  • The developed HCS platform using hiPSC-derived nociceptors offers a promising tool for rapid analgesic discovery.
  • This platform can significantly reduce the failure rate in preclinical-to-human translation for chronic pain treatments.
  • It facilitates the identification of novel therapeutic compounds targeting peripheral pain pathways.