Related Experiment Video
Updated: May 16, 2025
The Portable Chemical Sterilizer PCS, D-FENS, and D-FEND ALL: Novel Chlorine Dioxide Decontamination Technologies for the Military
Published on: June 29, 2014
Exploring the bactericidal efficacy of a new potassium monopersulphate-based disinfectant
P P Barbosa1, D M Leme2, N G Motta3
1School of Pharmacy and Pharmaceutical Sciences, Cardiff University, Cardiff, Wales, UK; University of Campinas (UNICAMP), Campinas, São Paulo, Brazil.
Background:
Staphylococcus aureus and Klebsiella pneumoniae are common pathogens responsible for hospital-acquired infections. Both species can survive on surfaces following desiccation and form dry surface biofilms (DSBs), which complicates the disinfection process.
Aim:
To evaluate the efficacy of an innovative potassium monopersulphate-based nanotechnology formulation (MPS) against both planktonic and sessile S. aureus and K. pneumoniae.
Methods:
The bactericidal efficacy of MPS was tested in comparison with sodium hypochlorite (NaOCl) and didecyldimethylammonium chloride (DDAC), which served as controls. The assessment was performed against planktonic bacteria, hydrated biofilm, and DSB using standard suspension and carrier tests. Scanning electron microscopy (SEM) was employed to identify any gross structural damage.
Findings:
MPS (2% w/v) achieved a ≥4 log10 reduction in K. pneumoniae with a short contact time, regardless of the test protocol. S. aureus was more resilient, but the introduction of wiping reduced the contact time needed to achieve a 4 log10 reduction from 15 to 5 min. SEM analysis revealed gross structural damage in both species following MPS treatment. The other disinfectants tested were also bactericidal, achieving ≥4 log10 reduction within 1-5 min, with the exception of DDAC against hydrated biofilms.
Conclusion:
The potassium monopersulphate-based formulation was found to be an effective bactericide, including against DSBs. Its efficacy compares favourably with other biocides commonly used in healthcare settings, and its biodegradability makes it a promising candidate for further development. However, optimization of the mechanical removal process will be essential to enhance MPS efficacy in practical applications.
Insights
A new potassium monopersulphate (MPS) nanotechnology formulation effectively kills Staphylococcus aureus and Klebsiella pneumoniae, including in dry surface biofilms (DSB). This biodegradable disinfectant shows promise for healthcare settings, though mechanical removal may enhance its efficacy.
Area of Science:
- Microbiology
- Infectious Diseases
- Nanotechnology
Background:
- * Staphylococcus aureus* and *Klebsiella pneumoniae* are leading causes of hospital-acquired infections.
- * Both pathogens form resilient dry surface biofilms (DSB), complicating disinfection protocols.
Purpose of the Study:
- * To assess the bactericidal effectiveness of a novel potassium monopersulphate (MPS) nanotechnology formulation.
- * To compare MPS efficacy against planktonic and sessile *S. aureus* and *K. pneumoniae* with standard disinfectants.
- * To evaluate MPS performance against dry surface biofilms (DSB).
Main Methods:
- * Standard suspension and carrier tests were used to evaluate bactericidal efficacy.
- * MPS was compared against sodium hypochlorite (NaOCl) and didecyldimethylammonium chloride (DDAC).
- * Scanning electron microscopy (SEM) was utilized to observe structural damage.
Main Results:
- * MPS (2% w/v) rapidly reduced *K. pneumoniae* by ≥4 log10.
- * *S. aureus* required wiping to achieve a ≥4 log10 reduction within 5 minutes.
- * SEM confirmed significant structural damage to both bacterial species post-treatment.
Conclusions:
- * The MPS formulation demonstrates potent bactericidal activity, including against DSB.
- * Its efficacy is comparable to existing healthcare biocides, with added benefits of biodegradability.
- * Optimizing mechanical removal is key for maximizing MPS effectiveness in real-world applications.
More Related Videos
11:47Evaluation of the Efficacy of Organic Peroxyacids for Eradicating Dairy Biofilms Using an Approach Combining Static and Dynamic Methods
Published on: December 9, 2022
06:36Establishing the Minimal Bactericidal Concentration of an Antimicrobial Agent for Planktonic Cells MBC-P and Biofilm Cells MBC-B
Published on: January 2, 2014
Related Concept Videos
01:23Hand hygiene
Hand washing...
01:30Cleaning, Sterilization, and Disinfection
Cleaning
The cleaning process usually involves using water with detergents or enzymatic cleaner and removing foreign material from objects and surfaces, including organic material such as body fluids or inorganic material like soil. Cleaning is performed before high-level disinfection and sterilization because foreign materials on the cover of the devices interfere with process...
01:30Methods of Sterilization II: Chemical Methods
Using chemical sterilization rather than heat to clean out equipment is recommended. It eradicates and removes all bacteria,...
01:29Methods of Sterilization I: Physical Methods
Steam sterilization uses non-toxic, low-cost moist heat in the form of saturated steam under pressure, which is fast, microbicidal, and sporicidal, and quickly warms and penetrates fabrics. Autoclaves, or steam sterilizers, expose each item to direct steam contact for a predetermined time at the necessary...