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Related Experiment Video

Updated: May 16, 2025

Author Spotlight: Improving Beef Cattle Nutrition and Production with a Focus on Feed Efficiency and Meat Quality Traits Through Advanced Biochemical and Molecular Assays
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Characterizing differences in the muscle transcriptome between cattle with alternative LCORL-NCAPG haplotypes.

Fernanda Martins Rodrigues1,2, Leif E Majeres3, Anna C Dilger1

  • 1Department of Animal Sciences, University of Illinois at Urbana-Champaign, Urbana, IL, USA.

BMC Genomics
|May 14, 2025
PubMed
Summary

Cattle with the QQ haplotype show increased lean growth due to altered muscle gene expression. This suggests muscle growth is favored over fat accumulation, potentially linked to LCORL gene expression differences.

Keywords:
LCORLBeefBovineFatMuscle

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Area of Science:

  • Animal Genetics
  • Molecular Biology
  • Livestock Science

Background:

  • The LCORL-NCAPG locus on bovine chromosome 6 (BTA6) significantly impacts cattle growth and carcass traits.
  • Understanding the molecular basis of these variations is crucial for livestock improvement.

Purpose of the Study:

  • To investigate the muscle transcriptome differences between cattle with alternative homozygous LCORL-NCAPG haplotypes (QQ vs. qq).
  • To elucidate the molecular mechanisms underlying enhanced growth and altered carcass composition associated with the QQ haplotype.

Main Methods:

  • Muscle biopsy collection from 24 Charolais-sired calves at 300 days of age.
  • RNA sequencing and gene expression analysis to compare QQ and qq individuals.
  • Gene set enrichment analysis to identify affected biological pathways.

Main Results:

  • 733 differentially expressed genes were identified between QQ and qq animals.
  • Upregulation of growth-related genes (e.g., LCORL, IGF2) and downregulation of adiposity-related genes (e.g., FASN, LEP) in QQ individuals.
  • QQ animals showed downregulated adipogenesis pathways and upregulated ribosomal and mitochondrial components, indicating enhanced protein synthesis and energy metabolism.

Conclusions:

  • The QQ haplotype favors muscle hypertrophy over fat accumulation, leading to increased lean growth.
  • Differences in LCORL expression may mediate downstream transcriptional effects and phenotypic changes.
  • Further research is needed to confirm the precise molecular mechanisms.