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Updated: May 17, 2025

Isolation of Murine Peritoneal Macrophages to Carry Out Gene Expression Analysis Upon Toll-like Receptors Stimulation
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Retinoic acid modulates peritoneal macrophage function and distribution to enhance antibacterial defense during

Yujuan Qin1,2, Xi Wang2,3, Xiamin Zhang1,4

  • 1Graduate School, Youjiang Medical University for Nationalities, Baise, China.

Frontiers in Nutrition
|May 15, 2025
PubMed
Abstract

Insights

Retinoic acid (RA) boosts macrophage immune defense against bacterial infections by enhancing phagocytosis and recruitment. RA-loaded nanoparticles offer sustained therapeutic effects for inflammation and infection management.

Area of Science:

  • Immunology
  • Cell Biology
  • Pharmacology

Background:

  • Peritoneal macrophages (LPMs and SPMs) are crucial for immune defense during inflammation.
  • Molecular mechanisms of macrophage response to retinoic acid (RA) are not fully understood.
  • Investigating RA's role in modulating macrophage function during bacterial infections.

Purpose of the Study:

  • Elucidate the role of RA in macrophage function, distribution, and immune responses.
  • Assess RA's impact on macrophage phagocytosis and population dynamics.
  • Explore sustained RA delivery using nanoparticle formulations.

Main Methods:

  • Established a murine model of peritonitis using Escherichia coli.
  • Assessed macrophage phagocytic capacity, population dynamics, and transcriptomic profiles.
  • Utilized immunofluorescence, flow cytometry, RNA sequencing, qPCR, and RA-loaded ZIF-8 nanoparticles.

Main Results:

  • RA significantly enhanced macrophage phagocytic activity and delayed functional decline.
  • RA promoted SPM recruitment and upregulated leukocyte migration and cell adhesion pathways.
  • RA-loaded nanoparticles demonstrated prolonged RA retention and sustained effects.

Conclusions:

  • RA enhances antibacterial defense by modulating macrophage activity.
  • RA influences distinct gene expression profiles in macrophage subpopulations.
  • RA and its nanoparticle formulations show therapeutic potential for bacterial infections and inflammation.
Keywords:
large peritoneal macrophagesnanoparticlesperitonitisretinoic acidsmall peritoneal macrophages

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