A Self-Circulated Microenvironment-Adaptive Nanozyme for Wound Healing Acceleration

Area of Science:

• Biomaterials Science
• Nanotechnology
• Wound Healing

Background:

• Nanozyme-based reactive oxygen species (ROS) homeostasis is crucial for infected wound healing.
• Nanozyme inactivation due to active center loss hinders sustained therapeutic effects.
• Achieving a balance between ROS production and scavenging in vivo remains challenging.

Purpose of the Study:

• To design a nanozyme with a regenerated active center for sustained therapeutic activity.
• To develop a nanozyme capable of controllable and reversible ROS generation and scavenging.
• To enhance infected wound healing through nanozyme-mediated ROS regulation and oxygen release.

Main Methods:

• Preparation of a novel nanozyme with a regenerated Fe(II) active center by adjusting ligands and metal charge transfer.
• Demonstration of cyclic and continuous oxidoreductase activities (peroxidase, superoxide dismutase, catalase, oxidase) at neutral pH.
• Evaluation of the nanozyme's effectiveness in controlling ROS, releasing oxygen, and eradicating bacteria in infected wounds.

Main Results:

• The developed nanozyme exhibits versatile enzymatic activity with a regenerated Fe(II) active center.
• Controllable and reversible ROS generation/scavenging and oxygen release were achieved.
• Ultralow dosage of the nanozyme demonstrated significant effectiveness against drug-resistant bacteria and accelerated wound healing (approx. 6.3 mm²/day).

Conclusions:

• The novel nanozyme design overcomes active center inactivation, providing sustained therapeutic benefits.
• This approach offers a promising strategy for advanced nanozyme-based infected wound management.
• The regenerated active center nanozyme represents a key breakthrough in wound healing applications.