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Updated: May 16, 2025

Author Spotlight: Development and Challenges of Feeding Assays for Insect Pest Management
04:51

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Published on: May 26, 2023

987

A Potent Plant-Derived Chitinase: Structural Informatics and Insecticidal Activity against Helicoverpa armigera.

Shah Rukh1, Ahmed Akrem2, Qamar Saeed3

  • 1Department of Biochemistry, Bahauddin Zakariya University, Multan, Pakistan.

Cell Biochemistry and Biophysics
|May 15, 2025
PubMed
Summary
This summary is machine-generated.

Nelumbo nucifera chitinase (NnChi) shows significant insecticidal activity against the cotton bollworm, Helicoverpa armigera. This plant-derived biopesticide offers a sustainable alternative to chemical pesticides, reducing agricultural losses.

Keywords:
Helicoverpa armigeraIn-silicoNelumbo nuciferaInsecticidal chitinaseMD simulationStructure prediction

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Area of Science:

  • Biochemistry
  • Molecular Biology
  • Entomology

Background:

  • Chemical pesticides for Helicoverpa armigera control cause environmental issues and resistance.
  • Plant-derived biopesticides are needed for sustainable pest management.
  • Nelumbo nucifera chitinase (NnChi) is explored as a potential biopesticide.

Purpose of the Study:

  • To elucidate the insecticidal potential of NnChi against Helicoverpa armigera.
  • To characterize the structural and functional aspects of NnChi.
  • To evaluate NnChi's efficacy through in-vivo bioassays.

Main Methods:

  • SDS-PAGE and LC-MS/MS for protein identification.
  • Structural informatics, including protein structure prediction and docking analysis.
  • Molecular dynamics (MD) simulations to study molecular interactions.
  • In-vivo insecticidal bioassays on H. armigera larvae.

Main Results:

  • NnChi protein identified with a molecular weight of ~32 kDa.
  • Docking analysis showed binding affinities of NnChi domains with (GlcNAc)4.
  • MD simulations revealed key residues involved in (GlcNAc)4 binding.
  • Significant larval mortality and adverse effects on life cycle parameters observed in H. armigera.

Conclusions:

  • NnChi exhibits significant insecticidal activity against Helicoverpa armigera.
  • Structural and functional characterization supports NnChi's insecticidal mechanism.
  • NnChi is a promising biopesticide candidate for sustainable pest control.