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Microfluidic Applications for Disposable Diagnostics

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Published on: February 3, 2008

Paper-Based Vertical Flow Assays for in Vitro Diagnostics and Environmental Monitoring.

Jaehyung Jeon^1,2, Heeseon Choi¹, Gyeo-Re Han²

¹School of Mechanical Engineering, Sungkyunkwan University (SKKU), Suwon 16419, Korea.

|May 15, 2025

Summary

Vertical flow paper-based analytical devices (VF-microPADs) enhance multiplexing for diagnostics and environmental monitoring. These devices overcome limitations of traditional microPADs, improving accuracy and speed for point-of-care applications.

Keywords:

Environmental monitoring In vitro diagnostics Multiplexing Paper-based analytical devices Point of care testing Vertical flow assay Vertical flow paper-based analytical devices

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Area of Science:

Biomedical Engineering
Analytical Chemistry
Materials Science

Background:

Microfluidic paper-based analytical devices (microPADs) offer portable and affordable diagnostics.
Conventional microPADs face limitations in multiplexing due to 2D fluid flow.
There is a need for advanced paper-based biosensors for complex analyses.

Purpose of the Study:

To review vertical flow paper-based analytical devices (VF-microPADs) and their advantages over conventional systems.
To explore the performance, fabrication, and applications of VF-microPADs.
To identify challenges and propose solutions for wider adoption of VF-microPADs.

Main Methods:

Review of existing literature on VF-microPADs and lateral flow systems.
Analysis of structural and functional performance trade-offs.
Discussion of sensing performance, fabrication techniques, and application data.

Main Results:

VF-microPADs enable axial fluid movement, enhancing multiplexing, reducing hook effect, and shortening assay times.
Comparison of VF-microPADs with lateral flow systems highlights performance differences.
Key applications in in vitro diagnostics and environmental monitoring are identified.

Conclusions:

VF-microPADs present significant advantages for simultaneous multibiomarker analysis.
Fabrication complexity, data analysis, and scalability are key challenges.
VF-microPADs hold potential for advancing point-of-care testing, especially in resource-limited settings.