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Humulus japonicus Enhances Bone Growth and Microarchitecture in Rats: Potential Involvement of IGF-1 Signaling
Seong-Hoo Park1, Jeongjin Park2, Jung-Yoon Yoo3
1Clinical Nutrition Institute, Kyung Hee University, Seoul, Korea.
Abstract:
This study investigated the effects of Humulus japonicus extract supplementation on bone growth and microstructural properties in male and female rats, with a particular focus on the JAK2/STAT5/IGF-1 signaling pathway. Three-week-old Sprague-Dawley rats were assigned to different groups receiving a normal diet (ND), growth hormone (GH), or varying doses of H. japonicus extract: low (L; 73 mg/kg body weight [bw]/day), medium (M; 146 mg/kg bw/day), and high (H; 292 mg/kg bw/day) for four weeks. The results demonstrated that the H group exhibited significant increases in femur and tibia lengths, trabecular and cortical bone mineral density, and growth plate thickness compared with the ND group. Furthermore, the H group demonstrated elevated serum and hepatic IGF-1 and IGFBP-3 levels, as well as enhanced phosphorylation of JAK2 and STAT5. The findings suggest that H. japonicus supplementation promotes longitudinal bone growth by stimulating growth plate activity and modulating the JAK2/STAT5-IGF-1 signaling pathway. This research indicates that H. japonicus extract could potentially be used as a natural therapeutic agent to support skeletal development and maintain bone health.
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