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Related Experiment Video

Updated: Jul 16, 2026

Development of Amelogenin-chitosan Hydrogel for In Vitro Enamel Regrowth with a Dense Interface

Development of Amelogenin-chitosan Hydrogel for In Vitro Enamel Regrowth with a Dense Interface

Published on: July 10, 2014

Bioinspired smart dentin ECM-chitosan hydrogels for dentin-pulp complex regeneration.

Maya Osman¹, Zinat Sharmin², Stephen Suchy²

¹College of Graduate Studies, Biomedical Sciences Program, Midwestern University, Downers Grove, IL, USA; College of Dental Medicine-Arizona, Midwestern University, Glendale, AZ, USA.

Journal of Dentistry

|May 15, 2025

Summary

New dentin ECM-chitosan hydrogels show promise for regenerating the dentin-pulp complex. These injectable scaffolds possess bioactivity, biocompatibility, and antimicrobial properties, offering a potential solution for dental caries treatment.

Keywords:

Antimicrobial Biocompatibility Bioinspired functional materials Chitosan Dentin extracellular matrix Scaffolds Smart hydrogels

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Last Updated: Jul 16, 2026

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Published on: May 5, 2023

Area of Science:

Biomaterials Science
Regenerative Dentistry
Tissue Engineering

Background:

Dental caries affects a significant portion of the global population, with treatments ranging from fillings to root canal therapy.
Current regenerative endodontic therapies lack scaffolds with inherent odontogenic potential and suitable microarchitecture.
Demineralized dentin extracellular matrix (ECM) offers unique physio-biological properties for scaffold development.

Purpose of the Study:

To develop and characterize novel hydrogels from demineralized dentin ECM and chitosan for dentin-pulp complex regeneration.
To evaluate the physicochemical, mechanical, and biological properties of these hydrogels.
To assess their potential as injectable scaffolds for regenerative dentistry.

Main Methods:

Dentin powder was partially demineralized and combined with chitosan in acetic acid solutions.
Hydrogel precursors were assessed for extrudability and gelation time.
Fabricated 3D constructs were characterized for spectral analysis, porosity, mechanical properties, biodegradability, bioactivity, antimicrobial activity, and biocompatibility.

Main Results:

The developed dentin ECM-chitosan hydrogels demonstrated suitable morphology and biomechanical attributes.
Both hydrogel formulations exhibited significant in-vitro bioactivity and biocompatibility.
Antimicrobial properties against Enterococcus faecalis were observed, along with cytocompatibility for dental pulp cells.

Conclusions:

Dentin ECM-chitosan hydrogels present a promising injectable scaffold for dentin-pulp complex regeneration.
These materials possess essential in-vitro bioactivity, biocompatibility, and antimicrobial characteristics.
The developed hydrogels offer a potential advancement in regenerative dentistry for treating dental caries.