Related Experiment Video
Updated: May 17, 2025
Contact Hypersensitivity as a Murine Model of Allergic Contact Dermatitis
Published on: September 26, 2022
Thresholds for Contact Allergy to a Rubber Accelerator: TETD-Determined by Serial Dilution Patch Testing and a
Christoffer Kursawe Larsen1,2,3, Jeanne D Johansen1,2,3, Claus Zachariae1,2,3
1National Allergy Research Centre, Department of Dermatology and Allergy, Herlev and Gentofte Hospital, Hellerup, Denmark.
Background:
Rubber accelerators found in rubber gloves may cause contact allergy and thus allergic contact dermatitis.
Objectives:
To investigate which doses of tetraethylthiuram disulfide (TETD) elicit allergic contact dermatitis by patch testing and in a repeated occluded application test simulating glove use.
Patients And Methods:
A multicentre study was conducted. Eight patients with contact allergy to TETD and eight controls comprised the study group. Doses of TETD 1% (400 μg/cm2), 0.33% (130 μg/cm2), 0.11% (44 μg/cm2), 0.037% (15 μg/cm2), 0.012% (4.9 μg/cm2), 0.0041% (1.6 μg/cm2) and pure vehicle (petrolatum) were used for the patch test. Doses of TETD 1% (39 μg/cm2), 0.33% (13 μg/cm2), 0.11% (4.3 μg/cm2) and pure vehicle (99:1 ethanol: water) were used for the repeated occluded application test.
Results:
Three patients (3/8) reacted to the lowest TETD dose of 0.0041% (1.6 μg/cm2) in the patch test. All patients reacted to all doses of TETD in the repeated occluded application test, none to the vehicle. The patch test dose of TETD that elicited allergic contact dermatitis in 10% of the patients (ED10) was estimated to be 0.75 μg/cm2.
Conclusion:
A content of 0.75 μg/cm2 TETD in rubber gloves may elicit allergic contact dermatitis. We suggest that future studies investigating contact allergy to rubber accelerators should base their methodology on the repeated occluded application test design presented in this study.
Trial Registration:
ClinicalTrial.gov identifier: NCT06042309.
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