Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Scanning Electron Microscopy01:07

Scanning Electron Microscopy

4.0K
A scanning electron microscope (SEM) is used to study the surface features of a sample by using an electron beam that scans the sample surface in a two-dimensional manner. Typically, areas between ~1 centimeter to 5 micrometers in width can be imaged. SEM can be used to image bacteria, viruses, tissues as well as larger samples like insects. Conventional SEM gives a magnification ranging from 20X to 30,000X and spatial resolution of 50 to 100 nanometers.
Fundamental Principles
Accelerated...
4.0K
Atomic Force Microscopy01:08

Atomic Force Microscopy

3.3K
Atomic force microscopy (AFM) is a type of scanning probe microscopy that can analyze topographic details of various specimens like ceramics, glass, polymers, and biological samples. AFM offers over 1000 times more resolution than the optical imaging system. Images generated from AFM are three-dimensional surface profiles, offering an advantage over the flat, two-dimensional images from other imaging techniques.
The AFM Probe
The probe is regarded as the heart of any AFM setup and comprises the...
3.3K
Overview of Microscopy Techniques01:22

Overview of Microscopy Techniques

9.6K
The early pioneers of microscopy opened a window into the invisible world of microorganisms. In 1830, Joseph Jackson Lister created an essentially modern light microscope. The 20th century saw the development of microscopes that leveraged nonvisible light, such as fluorescence microscopy that uses an ultraviolet light source and electron microscopy that uses short-wavelength electron beams. These advances significantly improved magnification, image resolution, and contrast. By comparison, the...
9.6K
Overview of Electron Microscopy01:25

Overview of Electron Microscopy

8.4K
The wavelengths of visible light ultimately limit the maximum theoretical resolution of images created by light microscopes. Most light microscopes can only magnify 1000X, and a few can magnify up to 1500X. Electrons, like electromagnetic radiation, can behave like waves, but with wavelengths of 0.005 nm, they produce significantly greater resolution up to 0.05 nm as compared to 500 nm for visible light. An electron microscope (EM) can create a sharp image that is magnified up to 2,000,000X.
8.4K
Transmission Electron Microscopy01:15

Transmission Electron Microscopy

5.3K
In 1931, physicist Ernst Ruska—building on the idea that magnetic fields can direct an electron beam just as lenses can direct a beam of light in an optical microscope—developed the first prototype of the electron microscope. This development led to the development of the field of electron microscopy. In the transmission electron microscope (TEM), electrons are produced by a hot tungsten element and accelerated by a potential difference in an electron gun, which gives them up to 400...
5.3K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Scaled Multidimensional Assays of Variant Effect Identify Sequence-Function Relationships in Hypertrophic Cardiomyopathy.

Circulation·2026
Same author

AutoMPP: Automated Task-Specific Multifingerprint Fusion for Property Prediction of Small Molecules and Macrocycles.

ChemMedChem·2026
Same author

Recent advances in molecular representation methods and their applications in scaffold hopping.

npj drug discovery·2026
Same author

Exploring chemical space based on Transformation to design broad-spectrum 3CL<sup>pro</sup> inhibitors against coronavirus.

European journal of medicinal chemistry·2026
Same author

Molecular glue degraders of HuR suppress BRAF-mutant colorectal cancer.

Nature·2026
Same author

Dual inhibition of KDM4B and KDM5A disassembles the PAX3-FOXO1 transcriptional program in fusion-positive rhabdomyosarcoma.

Biology direct·2026

Related Experiment Video

Updated: May 17, 2025

Precision Milling of Carbon Nanotube Forests Using Low Pressure Scanning Electron Microscopy
08:10

Precision Milling of Carbon Nanotube Forests Using Low Pressure Scanning Electron Microscopy

Published on: February 5, 2017

7.4K

Advancing cotton fiber research with variable-pressure scanning electron microscopy.

Fang Bai1, M Andrew Jansen2

  • 1The United States Department of Agriculture (USDA), Agricultural Research Service, Crop Genetics Research Unit, Stoneville, MS, United States.

Frontiers in Plant Science
|May 16, 2025
PubMed
Summary

Variable-pressure scanning electron microscopy (VP-SEM) offers a faster, cheaper, and more accurate method for observing cotton fiber development. This technique minimizes sample preparation and distortion, improving the study of these crucial textile resources.

Keywords:
cotton fiberfiber elongationfiber initiationmicroscopyscanning electron microscope (SEM)variable-pressure scanning electron microscope (VP-SEM)

More Related Videos

AFM-based Mapping of the Elastic Properties of Cell Walls: at Tissue, Cellular, and Subcellular Resolutions
10:26

AFM-based Mapping of the Elastic Properties of Cell Walls: at Tissue, Cellular, and Subcellular Resolutions

Published on: July 24, 2014

12.9K
Targeted Studies Using Serial Block Face and Focused Ion Beam Scan Electron Microscopy
09:09

Targeted Studies Using Serial Block Face and Focused Ion Beam Scan Electron Microscopy

Published on: August 10, 2019

9.1K

Related Experiment Videos

Last Updated: May 17, 2025

Precision Milling of Carbon Nanotube Forests Using Low Pressure Scanning Electron Microscopy
08:10

Precision Milling of Carbon Nanotube Forests Using Low Pressure Scanning Electron Microscopy

Published on: February 5, 2017

7.4K
AFM-based Mapping of the Elastic Properties of Cell Walls: at Tissue, Cellular, and Subcellular Resolutions
10:26

AFM-based Mapping of the Elastic Properties of Cell Walls: at Tissue, Cellular, and Subcellular Resolutions

Published on: July 24, 2014

12.9K
Targeted Studies Using Serial Block Face and Focused Ion Beam Scan Electron Microscopy
09:09

Targeted Studies Using Serial Block Face and Focused Ion Beam Scan Electron Microscopy

Published on: August 10, 2019

9.1K

Area of Science:

  • Plant biology
  • Textile science
  • Microscopy

Background:

  • Cotton fibers are vital renewable resources for textiles, originating from specialized epidermal cells.
  • Understanding cotton fiber development is key to improving yarn quality and fabric properties.
  • Traditional microscopy methods like scanning electron microscopy (SEM) are time-consuming and can distort samples.

Purpose of the Study:

  • To evaluate Variable-Pressure Scanning Electron Microscopy (VP-SEM) as an efficient alternative for studying early cotton fiber development.
  • To optimize VP-SEM imaging conditions for cotton fiber initiation and elongation.
  • To demonstrate the benefits of VP-SEM in reducing preparation time and sample artifacts.

Main Methods:

  • Application of VP-SEM to observe cotton fiber cell initiation and elongation in upland cotton (cultivar UGA 230) at 0 and 1-day post-anthesis.
  • Utilized ultra-variable-pressure and backscattered electron detectors for detailed imaging.
  • Identified optimal imaging parameters: 15 keV accelerating voltage and 50 Pa pressure.

Main Results:

  • VP-SEM enabled clear visualization of early fiber development stages with minimal sample preparation.
  • Optimized conditions (15 keV, 50 Pa) reduced image distortion and artifacts common in traditional SEM.
  • The protocol proved effective for imaging fresh biological samples, offering high-resolution insights.

Conclusions:

  • VP-SEM provides a high-resolution, cost-effective, and rapid method for studying cotton fiber development.
  • This technique minimizes sample damage and preparation time, enhancing research efficiency.
  • The optimized VP-SEM protocol is adaptable for diverse plant biology studies requiring real-time imaging.