Regulator of cullins-1 predicts a poor prognosis and regulates epithelial-mesenchymal transition process through GSK-3β/Wnt signaling in renal cell carcinoma
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View abstract on PubMed
Summary
This summary is machine-generated.Regulator of cullins-1 (ROC1) promotes renal cell carcinoma (RCC) progression by enhancing cell invasion and metastasis. ROC1 may serve as a therapeutic target for RCC treatment.
Area Of Science
- Oncology
- Molecular Biology
- Cancer Research
Background
- Renal cell carcinoma (RCC) is characterized by aggressive cell invasion and metastasis.
- The specific role of Regulator of Cullins-1 (ROC1) in RCC pathogenesis remains largely uncharacterized.
- This study investigates the biological impact and underlying mechanisms of ROC1 in RCC.
Purpose Of The Study
- To determine the expression levels of ROC1 in RCC tissues compared to normal tissues.
- To elucidate the functional role of ROC1 in RCC cell proliferation, invasion, and epithelial-mesenchymal transition (EMT).
- To explore the molecular pathway regulated by ROC1 in RCC, specifically the GSK-3β/Wnt signaling pathway.
Main Methods
- Messenger RNA (mRNA) and protein expression of ROC1 were analyzed using reverse transcription-polymerase chain reaction (RT-PCR) and western blot (WB) assays, respectively.
- Functional assays included Cell Counting Kit-8 (CCK-8), 5-ethynyl-2'-deoxyuridine (EdU) incorporation, Transwell invasion assays, and in vivo xenograft tumor models.
- The involvement of the GSK-3β/Wnt pathway was investigated through ROC1 manipulation and subsequent analysis.
Main Results
- ROC1 expression was significantly upregulated in RCC samples compared to healthy controls and correlated with poor patient outcomes.
- Overexpression of ROC1 enhanced RCC cell proliferation, EMT, and invasion, while ROC1 inhibition reversed these effects.
- ROC1 was found to regulate the GSK-3β/Wnt pathway, and its knockdown inhibited tumor metastasis in a mouse model, an effect partially reversed by shGSK-3β.
Conclusions
- ROC1 plays a tumor-promoting role in renal cell carcinoma.
- The findings suggest ROC1's involvement in regulating the GSK-3β/Wnt pathway contributes to RCC progression and metastasis.
- ROC1 represents a potential therapeutic target for managing renal cell carcinoma.
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