Morphological Characteristics and ITS Molecular Markers of Catharanthus roseus (L.) G. Don: A Medicinal Plant in Southern Vietnam
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View abstract on PubMed
Summary
This summary is machine-generated.This study confirms Catharanthus roseus samples from Southern Vietnam are genetically similar to the species, with 98.86-100% sequence identity. Findings support accurate identification and conservation of this valuable medicinal plant.
Area Of Science
- Botany
- Molecular Biology
- Pharmacognosy
Background
- Catharanthus roseus (L.) Don is a vital medicinal plant used for cancer and diabetes treatments.
- Accurate species identification through morphological and genetic markers is crucial for conservation and breeding.
- This research focuses on characterizing Catharanthus roseus in Southern Vietnam.
Purpose Of The Study
- To evaluate morphological features and molecular markers of Catharanthus roseus in Southern Vietnam.
- To enhance species characterization for improved medicinal utilization.
- To support conservation and breeding programs for Catharanthus roseus.
Main Methods
- Samples were analyzed using a completely randomized design with three replications.
- DNA extraction utilized an improved CTAB method.
- Internal Transcribed Spacer (ITS) gene region was amplified via PCR and sequenced using the Sanger method.
Main Results
- Sequence comparison of six Catharanthus roseus samples showed 98.86-100% similarity to the species on NCBI.
- Analysis of the ITS gene region and pedigree diagrams identified genera within the same family.
- High genetic similarity confirmed the identity of the studied samples.
Conclusions
- The studied samples were confirmed as Catharanthus roseus with high genetic similarity (98.86-100%).
- Future research should explore regional genetic diversity and bioactive compounds.
- This study supports the effective utilization and conservation of Catharanthus roseus.
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