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Updated: May 11, 2026
A Rapid and Specific Microplate Assay for the Determination of Intra- and Extracellular Ascorbate in Cultured Cells
Published on: April 11, 2014
Second-order advantage to quantify uric acid and ascorbic acid in blood serum by β-cyclodextrin inclusion
Abdollah Yari1, Saba Rashnoo1, Mansoureh Yari2
1Department of Chemistry, Lorestan University, Khorramabad, Iran.
Abstract:
The complexes formed between β-cyclodextrin (β-CD) and uric acid (UA) as well as ascorbic acid (AA) were examined utilizing parallel factor analysis (PFA) as a second-order calibration method (SOCM) for their quantification in blood serum samples. The second-order data were derived from the variations in spectral absorption changes associated with the β-CD complex signals. A three-dimensional dataset was created by stacking the data along the third dimension, which was analyzed by SOCM for determination of UA and AA. Validation of the method was achieved through comparison with HPLC. The findings indicated that the PFA method exhibited remarkable predictive capabilities. Figures of merit were calculated to highlight its potential as a viable alternative for the quantification of UA and AA, even in the presence of unforeseen or untemplated interferents, without necessitating any sample pretreatment.
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