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Inhibitors of Virion Maturation and Assembly

Inhibitors of Virion Maturation and Assembly

As part of their replication cycle, certain viruses synthesize long precursor proteins called polyproteins within infected host cells. In human immunodeficiency virus (HIV), two major polyproteins are produced: Gag and Gag-Pol. The Gag polyprotein supplies the structural components of the virus, while Gag-Pol includes essential viral enzymes such as reverse transcriptase, integrase, and protease. After synthesis, these polyproteins move to the host cell membrane, where they assemble into an...

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Related Experiment Video

Updated: Jun 22, 2026

Engineering and Evolution of Synthetic Adeno-Associated Virus AAV Gene Therapy Vectors via DNA Family Shuffling

Engineering and Evolution of Synthetic Adeno-Associated Virus AAV Gene Therapy Vectors via DNA Family Shuffling

Published on: April 2, 2012

CapBuild: a cloud-native tool for adeno-associated virus capsid engineering.

Anne H Klein¹, Michael J Kuiper², Mark Burgess³

¹Australian e-Health Research Centre, Commonwealth Scientific and Industrial Research Organisation (CSIRO), Herston, Queensland 4006, Australia.

Nucleic Acids Research

|May 16, 2025

Summary

This summary is machine-generated.

CapBuild is a new web server simplifying adeno-associated virus (AAV) capsid engineering for gene therapy. It enables researchers to easily predict, assemble, and design AAV capsids with high structural accuracy.

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Isolation of Next-Generation Gene Therapy Vectors through Engineering, Barcoding, and Screening of Adeno-Associated Virus AAV Capsid Variants

Isolation of Next-Generation Gene Therapy Vectors through Engineering, Barcoding, and Screening of Adeno-Associated Virus AAV Capsid Variants

Published on: October 18, 2022

Area of Science:

Biotechnology
Structural Biology
Bioinformatics

Background:

Adeno-associated virus (AAV) capsid engineering is crucial for gene therapy development.
Current methods face limitations due to structural complexity and computational demands.

Purpose of the Study:

To develop CapBuild, a cloud-native web server to streamline AAV capsid prediction, assembly, and engineering.
To provide accessible tools for rational AAV capsid design and structural analysis.

Main Methods:

Developed CapBuild with two workflows: PDB-based assembly and sequence-based modeling via SWISS-MODEL.
Integrated tools for icosahedral symmetry, mutation modeling, and amino acid localization.
Utilized Mol* for interactive 3D visualization of capsid structures.

Main Results:

Benchmarking against crystal structures showed high accuracy (0-0.89 Å RMSD, 89.4-100% GDT).
Demonstrated successful prediction, assembly, and visualization of AAV capsids.
Confirmed CapBuild's ability to facilitate site-specific mutation analysis and rational design.

Conclusions:

CapBuild significantly reduces technical barriers in AAV capsid engineering.
The platform enhances accessibility for researchers in early-stage AAV capsid design.
CapBuild empowers rational exploration and visualization of AAV structural variants for gene therapy applications.