Related Experiment Video
Updated: May 17, 2025

Live Cell Fluorescence Microscopy to Observe Essential Processes During Microbial Cell Growth
Published on: November 24, 2017
Constitutive Promoters Functional in Plant, Fungal, and Bacterial Hosts
Viktor V Morozov1, Anastasia V Balakireva2,1, Maxim M Perfilov2,1
1Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, Moscow 119991, Russia.
None:
Engineering of orthogonal systems functional across diverse hosts can benefit from employing universal regulatory DNA elements. Here, we screened a number of composite promoters in plant, fungal, and bacterial hosts, identifying variants that drive strong constitutive expression in Nicotiana tabacum, Saccharomyces cerevisiae, Escherichia coli, and Agrobacterium tumefaciens, or only in the eukaryotic subset of these organisms. These promoters can be used in universal vectors to co-optimize for different hosts in directed evolution, engineering of biosynthetic pathways, or other biotechnological tasks that require host switching.
More Related Videos
10:58Plant Promoter Analysis: Identification and Characterization of Root Nodule Specific Promoter in the Common Bean
Published on: December 23, 2017
08:51Applying an Inducible Expression System to Study Interference of Bacterial Virulence Factors with Intracellular Signaling
Published on: June 25, 2015
Related Concept Videos
Prokaryotic Transcriptional Activators and Repressors
Transcription of prokaryotic...
The Eukaryotic Promoter Region
Transgenic Plants
The first-ever transgenic plant was a tobacco plant developed in 1983 that showed resistance against the tobacco mosaic virus. Since then, many transgenic plants have been developed and commercialized for improving the agricultural, ornamental, and horticultural value of a crop plant. Transgenic...
Reporter Genes
Bacterial RNA Polymerase
In most genes, the transcription site is a single base present upstream of the coding sequence. Though RNAP is a catalytically efficient enzyme, it does not recognize...
Operons