Design and applications of photochromic compounds for quantitative chemical analysis and sensing

Photochromic compounds, capable of reversibly switching between distinct molecular states upon light irradiation, have emerged as powerful tools for quantitative chemical analysis and sensing. This feature reviews recent advancements in this developing field, focusing on the design principles and applications of photoswitchable sensors. We begin with a concise overview of the fundamental photophysics and photochromism of key compound classes, and then discuss the mechanisms of analyte recognition and signal transduction, showcasing how light-induced isomerization modulates analyte binding and enhances signal contrast compared to conventional optical sensors. The unique sensitivity of the photoswitching process to the microenvironment is also explored. Finally, we outline future research directions and challenges for realizing the full potential of photochromic compounds in analytical chemistry related fields, including diagnostics, environmental monitoring, and materials science.