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Microfluidic On-Chip Assay for Quantifying Blastoid Adhesion to Endometrial Epithelium
Aslı Ak1,2, Dorian Luijkx1, Daniel Carvalho1
1MERLN Institute for Technology-Inspired Regenerative Medicine, Maastricht University, Maastricht, The Netherlands.
Methods in Molecular Biology (Clifton, N.J.)
|May 16, 2025
Summary
Researchers developed a novel implantation-on-chip platform to precisely measure embryo attachment to the endometrium. This breakthrough aids in understanding early embryo implantation and developing treatments for infertility.
Area of Science:
- Reproductive Biology
- Biomedical Engineering
- Developmental Biology
Background:
- Endometrial receptivity is critical for successful embryo implantation, yet the factors influencing it are not fully understood.
- Current methods lack precise quantitative data on the initial stages of embryo implantation.
Purpose of the Study:
- To develop a microfluidic platform for quantifying functional embryo attachment to the endometrium.
- To assess endometrial epithelial receptivity using in vitro models.
Main Methods:
- Developed a personalized implantation-on-chip platform using endometrial organoids and blastoids.
- Cultured endometrial organoids into epithelial monolayers within microfluidic chips.
- Infused chips with blastoids and measured adhesion under controlled flow rates.
Main Results:
- Quantified functional embryo attachment using blastoid adhesion assays.
- Demonstrated a robust method for assessing endometrial epithelial receptivity.
- The platform allows for precise measurement of blastoid adhesion rates.
Conclusions:
- The developed microfluidic platform provides a robust method for studying endometrial receptivity.
- This technology can be used to test therapeutic interventions for infertility.
- Offers potential impact for infertile patients by improving understanding of implantation.
