Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Video

Updated: Jun 24, 2026

Quantification of Bacterial Histidine Kinase Autophosphorylation Using a Nitrocellulose Binding Assay
09:17

Quantification of Bacterial Histidine Kinase Autophosphorylation Using a Nitrocellulose Binding Assay

Published on: January 11, 2017

Complementary Fluorescent Probe Pair Targeting Histidinol Dehydrogenase Provides a Useful Tool for Target Validation.

Wu-Yingzheng Guo1, Yi-Xuan Fu1, Stephen Smith2

  • 1State Key Laboratory of Green Pesticide, Central China Normal University, Wuhan 430079, P. R. China.

Analytical Chemistry
|May 16, 2025
PubMed
Summary

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Unique Imaging Findings of a Calvarial Ossifying Fibroma Successfully Managed With Surgery and Definitive Intent Radiotherapy.

Veterinary radiology & ultrasound : the official journal of the American College of Veterinary Radiology and the International Veterinary Radiology Association·2026
Same author

Photoinduced Radical Epoxidation of <i>N</i>-Alkoxyphthalimides with Allylic Peroxides.

Organic letters·2026
Same author

Photoelectrocatalysis-Enabled C(sp<sup>3</sup>)-C(sp<sup>3</sup>) Cross-Coupling of Carboxylic Acids with Alkyl Halides.

Journal of the American Chemical Society·2026
Same author

Design, Synthesis, and Bioactivity of 2,4-Diphenylquinoline Derivatives as Novel 1-Deoxy-d-xylulose-5-phosphate Synthase Inhibitors.

Journal of agricultural and food chemistry·2026
Same author

Trehalose 6-Phosphate Signaling for Sustainable Crop Improvements.

Journal of agricultural and food chemistry·2026
Same author

Enhancing conventional biopsies: ultrasound-guided percutaneous bone marrow biopsy and aspiration.

Journal of ultrasound·2026

Developing new pesticides targeting histidinol dehydrogenase (HDH) is challenging. Novel fluorescent probes HDP1 and HDP2 reveal that HDH inhibitors compete with natural substrates and that stress affects histidine biosynthesis, complicating pesticide development.

Area of Science:

  • Agrochemical research
  • Molecular biology
  • Biochemistry

Background:

  • Target-based pesticide design requires validation of novel candidate targets.
  • Fluorescent probes are essential for studying molecular interactions and target functionality.

Purpose of the Study:

  • To develop and utilize a fluorescent probe pair (HDP1 and HDP2) to assess the agrochemical potential of targeting histidinol dehydrogenase (HDH).
  • To investigate the interactions between HDH, its natural substrate (HOL), and inhibitors.
  • To visualize HDH activity and histidine biosynthesis pathway flux in vivo under various stress conditions.

Main Methods:

  • Development of a complementary fluorescent probe pair, HDP1 and HDP2.
  • Using HDP1 to probe inhibitor and substrate interactions with HDH.

More Related Videos

Assays for Validating Histone Acetyltransferase Inhibitors
09:11

Assays for Validating Histone Acetyltransferase Inhibitors

Published on: August 6, 2020

An Acetyl-Click Chemistry Assay to Measure Histone Acetyltransferase 1 Acetylation
05:44

An Acetyl-Click Chemistry Assay to Measure Histone Acetyltransferase 1 Acetylation

Published on: January 26, 2024

Related Experiment Videos

Last Updated: Jun 24, 2026

Quantification of Bacterial Histidine Kinase Autophosphorylation Using a Nitrocellulose Binding Assay
09:17

Quantification of Bacterial Histidine Kinase Autophosphorylation Using a Nitrocellulose Binding Assay

Published on: January 11, 2017

Assays for Validating Histone Acetyltransferase Inhibitors
09:11

Assays for Validating Histone Acetyltransferase Inhibitors

Published on: August 6, 2020

An Acetyl-Click Chemistry Assay to Measure Histone Acetyltransferase 1 Acetylation
05:44

An Acetyl-Click Chemistry Assay to Measure Histone Acetyltransferase 1 Acetylation

Published on: January 26, 2024

  • Employing HDP2 for in vivo imaging of HDH in Arabidopsis thaliana, Escherichia coli, and Saccharomyces cerevisiae.
  • Analyzing changes in histidine biosynthesis pathway flux under stress and pesticide treatment.

    • Main Results:

    • HDP1 demonstrated high sensitivity for detecting HDH interactions (0.17 microg/mL detection limit).
    • HDP2 provided excellent in vivo imaging of HDH.
    • HOL, the natural substrate, competitively binds to HDH, similar to HDH inhibitors.
    • HDH inhibitors, other pesticides, and abiotic stresses impact histidine biosynthesis across different organisms.
    • Stresses affect histidine biosynthesis via pentose phosphate pathway regulation and ATP-phosphoribosyltransferase expression inhibition.

    Conclusions:

    • Developing effective in vivo HDH inhibitors that compete with HOL remains a significant challenge.
    • The histidine biosynthesis pathway's sensitivity to various stresses complicates HDH-targeted pesticide development.
    • Environmental and cellular stresses can modulate the effectiveness of synthetic ligands targeting HDH.