Ratiometric fluorescent probes based on fluorogenic reactions of o-phenylenediamine for multiple sensing...

Area of Science:

• Analytical Chemistry
• Materials Science
• Chemical Sensing

Background:

• Ratiometric fluorescent probes offer advantages in sensing due to internal referencing, reducing environmental interference.
• The in-situ fluorescence reaction of o-phenylenediamine (OPD) provides a convenient and efficient platform for probe construction.
• A systematic review of OPD-based ratiometric probe design strategies and applications is needed to guide future development.

Purpose of the Study:

• To systematically review and categorize the design strategies of ratiometric fluorescent probes based on the in-situ fluorescence reaction of o-phenylenediamine (OPD).
• To summarize the diverse applications of these OPD-based ratiometric probes.
• To provide insights for the development of multifunctional ratiometric fluorescent probes.

Main Methods:

• Categorization of ratiometric fluorescent probe construction based on the in-situ reaction of OPD into three main types.
• Classification based on the generated fluorescent products: yellow, blue, and combined blue-yellow.
• Comprehensive review of reported applications for each probe category.

Main Results:

• Construction strategies for OPD-based ratiometric fluorescent probes are classified into three categories: yellow fluorescence, blue fluorescence, and combined blue-yellow fluorescence products.
• Each category demonstrates distinct sensing capabilities and applications.
• The review consolidates knowledge on probe design and application, highlighting versatility.

Conclusions:

• The in-situ fluorescence reaction of o-phenylenediamine (OPD) is a versatile strategy for constructing ratiometric fluorescent probes.
• Categorizing probes by their fluorescence output (yellow, blue, combined) aids in understanding their design and application scope.
• This review provides a foundation for developing advanced, multifunctional ratiometric fluorescent probes for broader scientific fields.