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Related Experiment Video

Updated: Jun 1, 2026

In Vitro Differentiation of Human Pluripotent Stem Cells into Trophoblastic Cells

In Vitro Differentiation of Human Pluripotent Stem Cells into Trophoblastic Cells

Published on: March 16, 2017

Post-gastrulation amnioids as a stem cell-derived model of human extra-embryonic development.

Borzo Gharibi¹, Oliver C K Inge¹, Irene Rodriguez-Hernandez²

¹Quantitative Stem Cell Biology Laboratory, The Francis Crick Institute, London NW1 1AT, UK.

|May 16, 2025

Summary

Researchers developed post-gastrulation amnioids (PGAs), a 3D stem cell model for studying human amnion development. This model reveals GATA3

Keywords:

BMP4 GATA3 WNT amnion extra-embryonic tissues hESCs human development self-organization stem cell-based models of human development

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Last Updated: Jun 1, 2026

In Vitro Differentiation of Human Pluripotent Stem Cells into Trophoblastic Cells

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Published on: November 8, 2024

Area of Science:

Developmental Biology
Stem Cell Biology
Reproductive Biology

Background:

The amnion is vital for mammalian embryonic development, providing essential support.
Studying human amnion formation and function is challenging due to limited in vitro models.

Purpose of the Study:

To develop a novel in vitro model for studying human amnion development.
To investigate the molecular mechanisms governing amniogenesis.

Main Methods:

Generation of a 3D embryonic stem cell-derived model of post-gastrulation amnioids (PGAs).
Recapitulation of extra-embryonic development up to 4 weeks post-fertilization.
Analysis of GATA3's role in amniogenesis.

Main Results:

PGAs accurately mimic human amniotic sac development and function.
GATA3 was identified as both necessary and sufficient for amniogenesis.
An autoregulatory feedback loop involving extra-embryonic signals was found to regulate amnion formation.

Conclusions:

The PGA model offers a reproducible and scalable platform for studying embryo-amnion interactions.
This model facilitates research into early pregnancy development and potential clinical applications.
The findings elucidate key regulatory mechanisms of amnion specification.