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Molecular characterization of a novel mitovirus from the edible fungus Pleurotus pulmonarius

Zhe Wang1, Junbo Xiao1, Hanzhao Liu1

  • 1State Key Laboratory of Green Pesticide, College of Plant Protection, South China Agricultural University, Guangzhou, 510642, China.

Archives of Virology
|May 16, 2025
PubMed

Insights

Researchers discovered a new mitovirus, Pleurotus pulmonarius duamitovirus 1 (PpDMV1), in the edible fungus Pleurotus pulmonarius. This marks the first documented mitovirus infection in this species.

Area of Science:

  • Mycology
  • Virology
  • Fungal Genetics

Background:

  • Mitoviruses are non-segmented, single-stranded positive-sense RNA viruses that infect fungi.
  • Pleurotus pulmonarius is a widely consumed edible mushroom species.
  • Understanding fungal viromes is crucial for agricultural and food security.

Purpose of the Study:

  • To identify and characterize novel viruses in Pleurotus pulmonarius.
  • To determine the phylogenetic relationship of the newly identified virus.

Main Methods:

  • Fungal sample collection and nucleic acid extraction.
  • Whole-genome sequencing and assembly.
  • Bioinformatic analysis including BLASTx and phylogenetic tree construction.

Main Results:

  • Identification of a novel mitovirus, Pleurotus pulmonarius duamitovirus 1 (PpDMV1).
  • PpDMV1 possesses a 2,622-nucleotide genome with a single open reading frame encoding an RNA-dependent RNA polymerase.
  • Phylogenetic analysis places PpDMV1 within the Duamitovirus genus, with closest similarity to EnMV15.

Conclusions:

  • This study reports the first mitovirus identified in Pleurotus pulmonarius.
  • PpDMV1 represents a new member of the Duamitovirus genus.
  • The discovery expands our knowledge of fungal virus diversity.

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