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Updated: Jul 16, 2026

A Bioinformatics Pipeline to Accurately and Efficiently Analyze the MicroRNA Transcriptomes in Plants
06:34

A Bioinformatics Pipeline to Accurately and Efficiently Analyze the MicroRNA Transcriptomes in Plants

Published on: January 21, 2020

In Vitro Transcription of Plant miRNA for Structural and Processing Analysis

Corina Diaz-Quezada1, Noe Baruch-Torres2, Carlos H Trasviña-Arenas3

  • 1Unidad de Genomica Avanzada (UGA), Centro de Investigación y de Estudios Avanzados del Instituto Politécnico Nacional (Cinvestav), Carretera Irapuato-León, Irapuato, Guanajuato, Mexico.

Methods in Molecular Biology (Clifton, N.J.)
|May 16, 2025
PubMed

Keywords:
Denaturing gel electrophoresisFootprintingProcessingSecondary structureT7 RNA polymerasemiRNApri-miRNA

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MicroRNA (miRNA) are short, regulatory RNA transcribed from introns—non-coding regions of a gene—or intergenic regions—stretches of DNA present between genes. Several processing steps are required to form biologically active, mature miRNA. The initial transcript, called primary miRNA (pri-mRNA), base-pairs with itself forming a stem-loop structure. Within the nucleus, an endonuclease enzyme, called Drosha, shortens the stem-loop structure into hairpin-shaped pre-miRNA. After the pre-miRNA ends...
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MicroRNA (miRNA) are short, regulatory RNA transcribed from introns (non-coding regions of a gene) or intergenic regions (stretches of DNA present between genes). Several processing steps are required to form biologically active, mature miRNA. The initial transcript, called primary miRNA (pri-mRNA), base-pairs with itself, forming a stem-loop structure. Within the nucleus, an endonuclease enzyme, called Drosha, shortens the stem-loop structure into hairpin-shaped pre-miRNA. After the pre-miRNA...