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Updated: Jul 15, 2026

Potato Virus X-Based microRNA Silencing (VbMS) In Potato.
Published on: May 11, 2020
Gene Silencing in Plants Through Exogenous Application of miRNAs
Yunuen Quetzali Garduño-Tamayo1, Gerardo Acosta-García2
1Departamento de Ingeniería Bioquímica, Instituto Tecnológico de Celaya, Celaya, Guanajuato, Mexico.
Abstract:
Although omics technologies allow us to identify genes involved in various biological processes, we still rely on the analysis of the function of individual genes. Studies of the function of a specific gene include technologies such as gene silencing by RNAi or genome editing by CRISPR-cas9. However, most of them depend on the availability of transformation methods, so they are not established for all plants. In this chapter, we report a protocol that involves the exogenous application of miRNAs for the specific silencing of genes of interest. The advantage of this protocol is that it does not require a transformation event and can be applied to a certain tissue or developmental stage. This novel technology facilitates the analysis of specific gene functions in crops of economic interest.
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