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Related Concept Videos

Ribosomes01:27

Ribosomes

Ribosomes translate genetic information encoded by messenger RNA (mRNA) into proteins. Both prokaryotic and eukaryotic cells have ribosomes. Cells that synthesize large quantities of protein—such as secretory cells in the human pancreas—can contain millions of ribosomes.Ribosome Structure and AssemblyRibosomes are composed of ribosomal RNA (rRNA) and proteins. In eukaryotes, rRNA is transcribed from genes in the nucleolus—a part of the nucleus that specializes in ribosome production. Within the...
Ribosomes01:27

Ribosomes

Ribosomes translate genetic information encoded by messenger RNA (mRNA) into proteins. Both prokaryotic and eukaryotic cells have ribosomes. Cells that synthesize large quantities of protein—such as secretory cells in the human pancreas—can contain millions of ribosomes.Ribosome Structure and AssemblyRibosomes are composed of ribosomal RNA (rRNA) and proteins. In eukaryotes, rRNA is transcribed from genes in the nucleolus—a part of the nucleus that specializes in ribosome production. Within the...
Ribosome Profiling02:24

Ribosome Profiling

Ribosome profiling or ribo-sequencing is a deep sequencing technique that produces a snapshot of active translation in a cell. It selectively sequences the mRNAs protected by ribosomes to get an insight into a cell’s translation landscape at any given point in time.
Applications of ribosome profiling
Ribosome profiling has many applications, including in vivo monitoring of translation inside a particular organ or tissue type and quantifying new protein synthesis levels.
The technique helps...
Ribosomal RNA Synthesis02:53

Ribosomal RNA Synthesis

Ribosome synthesis is a highly complex and coordinated process involving more than 200 assembly factors. The synthesis and processing of ribosomal components occurs not only in the nucleolus but also in the nucleoplasm and the cytoplasm of eukaryotic cells.
Ribosome biogenesis begins with the synthesis of 5S and 45S pre-rRNAs by distinct RNA polymerases. The primary transcripts are extensively processed and modified before they are bound and folded by ribosomal proteins and assembly factors,...
Ribosomes01:27

Ribosomes

Ribosomes translate genetic information encoded by messenger RNA (mRNA) into proteins. Both prokaryotic and eukaryotic cells have ribosomes. Cells that synthesize large quantities of protein—such as secretory cells in the human pancreas—can contain millions of ribosomes.
Ribosome Structure and Assembly
Ribosomes are composed of ribosomal RNA (rRNA) and proteins. In eukaryotes, rRNA is transcribed from genes in the nucleolus—a part of the nucleus that specializes in ribosome production. Within...
Ribosomes01:27

Ribosomes

Ribosomes translate genetic information encoded by messenger RNA (mRNA) into proteins. Both prokaryotic and eukaryotic cells have ribosomes. Cells that synthesize large quantities of protein—such as secretory cells in the human pancreas—can contain millions of ribosomes.
Ribosome Structure and Assembly
Ribosomes are composed of ribosomal RNA (rRNA) and proteins. In eukaryotes, rRNA is transcribed from genes in the nucleolus—a part of the nucleus that specializes in ribosome production. Within...

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Related Experiment Video

Updated: Jun 19, 2026

Polysome Fractionation and Analysis of Mammalian Translatomes on a Genome-wide Scale
10:56

Polysome Fractionation and Analysis of Mammalian Translatomes on a Genome-wide Scale

Published on: May 17, 2014

Advances in ribosome profiling technologies.

Kotaro Tomuro1,2, Shintaro Iwasaki1,2

  • 1RNA Systems Biochemistry Laboratory, Pioneering Research Institute, RIKEN, Wako, Saitama 351-0198, Japan.

Biochemical Society Transactions
|May 17, 2025
PubMed
Summary
This summary is machine-generated.

Ribosome profiling (Ribo-seq) reveals protein synthesis regulation using ribosome footprints. Recent Ribo-seq innovations enhance sensitivity and resolution for detailed translational control insights.

Keywords:
deep sequencingribo-seqribosome profilingtranslational control

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RIBO-seq in Bacteria: a Sample Collection and Library Preparation Protocol for NGS Sequencing

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De novo Identification of Actively Translated Open Reading Frames with Ribosome Profiling Data
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De novo Identification of Actively Translated Open Reading Frames with Ribosome Profiling Data

Published on: February 18, 2022

Related Experiment Videos

Last Updated: Jun 19, 2026

Polysome Fractionation and Analysis of Mammalian Translatomes on a Genome-wide Scale
10:56

Polysome Fractionation and Analysis of Mammalian Translatomes on a Genome-wide Scale

Published on: May 17, 2014

RIBO-seq in Bacteria: a Sample Collection and Library Preparation Protocol for NGS Sequencing
12:05

RIBO-seq in Bacteria: a Sample Collection and Library Preparation Protocol for NGS Sequencing

Published on: August 7, 2021

De novo Identification of Actively Translated Open Reading Frames with Ribosome Profiling Data
08:23

De novo Identification of Actively Translated Open Reading Frames with Ribosome Profiling Data

Published on: February 18, 2022

Area of Science:

  • Molecular Biology
  • Genomics
  • Proteomics

Background:

  • Ribosome profiling (Ribo-seq) is a key technique for studying protein synthesis.
  • Deep sequencing of ribosome footprints provides insights into translational regulation.
  • Advances in Ribo-seq have improved its sensitivity, specificity, and resolution.

Purpose of the Study:

  • To review emerging Ribo-seq derivatives and their applications.
  • To highlight innovations overcoming technical limitations in Ribo-seq.
  • To showcase Ribo-seq's utility in understanding translational control.

Main Methods:

  • Review of recent literature on Ribo-seq technologies.
  • Analysis of Ribo-seq derivatives addressing specific challenges.
  • Discussion of applications in diverse biological contexts.

Main Results:

  • Emerging Ribo-seq methods address challenges like low input, rRNA contamination, and data calibration.
  • New Ribo-seq techniques support single-cell applications.
  • These advances provide deeper insights into translational regulation.

Conclusions:

  • Ribo-seq is a powerful and evolving tool for studying protein synthesis.
  • Innovations in Ribo-seq are expanding its scope and applicability.
  • Ribo-seq derivatives offer enhanced capabilities for dissecting translational control.