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Biological Cascade Catalysts Based on Structurally Regulated Covalent Organic Framework for Intuitive Glucose
Tong Lu1, Shuanglong Lu1, Hongze Yao2
1Key Laboratory of Synthetic and Biological Colloids, Ministry of Education, School of Chemical and Material Engineering, Jiangnan University, Wuxi 214122, P. R. China.
ACS Applied Materials & Interfaces
|May 17, 2025
Summary
This study introduces Fe-ion-coordinated covalent organic frameworks (Fe-COFs) as efficient carriers for immobilizing glucose oxidase (GOx). These novel nanozyme-based sensors enable sensitive and convenient glucose detection, expanding biocatalysis applications in medical diagnostics.
Area of Science:
- Materials Science
- Biocatalysis
- Nanotechnology
- Chemical Engineering
Background:
- Catalytic cascade reactions integrating chemical and biological catalysts show promise but face challenges like enzyme fragility and low efficiency.
- Covalent organic frameworks (COFs) offer potential as carriers, but their application in biocatalysis requires tailored properties.
- Developing robust and efficient immobilization strategies is crucial for practical biocatalytic applications.
Purpose of the Study:
- To synthesize and characterize novel Fe-ion-coordinated covalent organic frameworks (Fe-COFs) as carriers for enzyme immobilization.
- To evaluate the suitability of Fe-COFs for immobilizing glucose oxidase (GOx) in cascade catalytic systems.
- To develop a smartphone-compatible colorimetric sensor for sensitive glucose detection using Fe-COF-immobilized GOx.
Main Methods:
- One-pot synthesis of two types of Fe-COFs with similar topologies but different electronic structures.
- Immobilization of glucose oxidase (GOx) onto the synthesized Fe-COFs.
- Development and validation of a colorimetric sensor for glucose quantification using Fe-COF/GOx nanozymes.
Main Results:
- Fe-COFs exhibited excellent peroxidase activity without oxidase interference, enabling high GOx loading capacity (>0.9 mg/mgCOF).
- The optimized Fe-COF1 carrier facilitated the development of a sensitive colorimetric sensor for trace glucose detection (LoD: 1.4 μM).
- The sensor demonstrated excellent stability (90% activity after 10 cycles) and efficacy in detecting glucose in serum samples.
Conclusions:
- Fe-COFs are effective and robust carriers for GOx immobilization, overcoming limitations of traditional carriers.
- The developed nanoenzyme-based sensor offers a convenient and sensitive platform for glucose quantification and medical diagnostics.
- This work highlights the potential of COF-based biocatalysts in advancing colorimetric sensing and point-of-care applications.
