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Updated: May 20, 2025

Motility of Single Molecules and Clusters of Bi-Directional Kinesin-5 Cin8 Purified from S. cerevisiae Cells
Published on: February 2, 2022
Structure-guided development of Quinoline derivatives targeting kinesin spindle protein
Rohini S Kavalapure1, Shankar G Alegaon1, Shankar Gharge1
1Department of Pharmaceutical Chemistry, KLE College of Pharmacy, Belagavi, KLE Academy of Higher education and Research, Belagavi 590 010, Karnataka, India.
None:
The kinesin Eg5 protein is a promising target for cancer therapy due to its crucial role in mitosis. This study designed and evaluated 2-((7-chloroquinolin-4-yl) amino) benzohydrazide derivatives as Eg5 inhibitors. Compounds 6d and 6e exhibited potent inhibition (IC50: 1.519 ± 0.4415 microM and 0.2848 ± 0.070 microM, respectively) and significant antiproliferative activity against MCF-7 cells. Pharmacophore modeling, docking, MD simulations, and MM/GBSA analyses confirmed stable interactions within the Eg5 active site. These compounds also modulate breast cancer-related pathways, including PI3K-Akt and MAPK. These findings highlight compounds 6d and 6e as promising anticancer agents, warranting further in vivo studies to validate their therapeutic potential.
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