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Genome-based alert on a clinical Plesiomonas shigelloides PSU59 from Thailand: Resistance and virulence features

Thunchanok Yaikhan1, Kamonnut Singkhamanan1, Thitaporn Dechathai1

  • 1Department of Biomedical Sciences and Biomedical Engineering, Faculty of Medicine, Prince of Songkla University, Songkhla 90110, Thailand.

Infection, Genetics and Evolution : Journal of Molecular Epidemiology and Evolutionary Genetics in Infectious Diseases
|May 17, 2025
PubMed

Insights

Plesiomonas shigelloides PSU59, a bacterium from Thailand, shows potential as an emerging pathogen. Genomic analysis revealed antimicrobial resistance genes and virulence factors, highlighting the need for surveillance.

Area of Science:

  • Microbiology
  • Genomics
  • Infectious Diseases

Background:

  • Plesiomonas shigelloides is an aquatic bacterium gaining recognition as an emerging pathogen.
  • Antimicrobial resistance (AMR) is a growing concern in P. shigelloides infections.

Purpose of the Study:

  • To perform a genome-based analysis of P. shigelloides PSU59, isolated from a patient in Thailand.
  • To identify AMR and virulence factors in the P. shigelloides PSU59 genome.

Main Methods:

  • Whole-genome sequencing (WGS) of P. shigelloides PSU59.
  • Bioinformatic analysis to identify coding sequences, RNA genes, AMR genes, and virulence factors.
  • Phylogenetic comparison with other P. shigelloides strains.

Main Results:

  • A 3.6 Mb draft genome was sequenced, containing 3265 coding sequences and 129 RNA genes.
  • Thirteen AMR genes, including efflux pumps and target modifiers, were identified.
  • Forty-eight virulence factors, including flagellar genes associated with motility, were detected. Mobile genetic elements suggest horizontal gene transfer.

Conclusions:

  • P. shigelloides PSU59 possesses significant pathogenic and drug-resistant potential.
  • Genomic surveillance is crucial for monitoring emerging threats from under-recognized pathogens like P. shigelloides.
Keywords:
And phylogenetic diversityAntimicrobial resistance (AMR)Mobile genetic elements (MGEs)Plesiomonas shigelloides

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