A novel approach in reducing hypercholesterolemia using modified mango kernel starch and its role as a potential fat...

The global burden of hypercholesterolemia is approximately 2.6 million deaths and 29.7 million disability adjusted life years. Using modified mango kernel starch to manage and prevent hypercholesterolemia has garnered international interest. According to growing data, starch treated with acid has been shown to enhance lipid metabolism by reducing the concentration of low-density lipoprotein cholesterol in the plasma of hypercholesterolemic patients. The present study aims to determine the effect of modified mango kernel starch on the hypercholesterolemic mice model. Mango kernel starches harvested from different species of Mangifera indica. Raw starches were then subjected to chemical modification using acid hydrolysis method. An in-vivo hypercholesterolemic model was developed using C57BL/6 to investigate the effect of chemically modified starch. Acid hydrolysis treatment in mango kernel starch resulted in a considerable increase in water-holding capacity, amylose content without affecting oil-binding capacities. FTIR spectra report revealed interactions between starch chains in the modified and native starches. SEM image showed the changes in surface micrographs of modified mango kernel starch. Modified mango kernel starch significantly improved total cholesterol, triglycerides, and lipid peroxidation in hypercholesterolemic mice. Furthermore, the administration of modified mango kernel starch markedly impeded the rise in plasma TNF-α and IL-6 levels. Modified mango kernel starch treated animals showed reduced expression of cholesterol-related proteins in the liver, including FAS, ACC, and SREBP1. All the results indicate that modified mango kernel starch can be an excellent alternative for therapeutic intervention of hypercholesterolemic individuals with the potential to act as a fat replacer.