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Updated: May 20, 2025

Preparation and In Vivo Use of an Activity-based Probe for N-acylethanolamine Acid Amidase
Published on: November 23, 2016
Measurements of acrolein adducts resulting from polyamine catabolism
Keiko Kashiwagi1, Kazuei Igarashi1
1Amine Pharma Research Institute, Innovation Plaza at Chiba University, Chiba, Japan.
Abstract:
Acrolein (CH2=CH-CHO) is an unsaturated aldehyde, produced primarily from spermine [NH2(CH2)3NH(CH2)4NH(CH2)3NH2] by spermine oxidase (SMOX), and shows high reactivity with protein cysteine- and lysine-residues to produce protein-conjugated acrolein (PC-Acro) or amino acid-conjugated acrolein (AC-Acro). Increase in PC-Acro in plasma has been identified as a biomarker for stroke, silent brain infarction (SBI) and dementia such as Alzheimer's disease. In contrast, decrease in AC-Acro and taurine in urine was observed in dementia. In addition, properties of proteins such as glyceraldehyde 3-phosphate dehydrogenase (GAPDH) and cytoskeleton proteins are altered by conjugation with acrolein.
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