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Related Concept Videos

RNA-seq03:21

RNA-seq

RNA sequencing, or RNA-Seq, is a high-throughput sequencing technology used to study the transcriptome of a cell. Transcriptomics helps to interpret the functional elements of a genome and identify the molecular constituents of an organism. Additionally, it also helps in understanding the development of an organism and the occurrence of diseases. 
Before the discovery of RNA-seq, microarray-based methods and Sanger sequencing were used for transcriptome analysis. However, while microarray-based...

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Transcriptome data from the interdigital webs of the Chinese Soft-shell Turtle (Pelodiscus sinensis).

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  • 1College of Animal Science and Veterinary Medicine, Shenyang Agricultural University, Shenyang, 110866, China.

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|May 17, 2025
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Researchers studied gene expression in Chinese soft-shell turtle web development to understand how aquatic adaptations evolve. This research sheds light on limb morphogenesis and the regulation of interdigital cell death.

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Area of Science:

  • Developmental Biology
  • Evolutionary Biology
  • Comparative Genomics

Background:

  • Interdigital webbing in aquatic tetrapods is crucial for adaptation and arises from inhibited interdigital cell death (ICD).
  • Diverse web morphologies have evolved independently, but the underlying regulatory mechanisms remain unclear.
  • The Chinese soft-shell turtle (Pelodiscus sinensis) displays transitional webbing, making it an ideal model organism.

Purpose of the Study:

  • To investigate the molecular mechanisms governing the development of interdigital webbing in Pelodiscus sinensis.
  • To identify key genes involved in the regulation of interdigital cell death (ICD) during limb morphogenesis.
  • To provide insights into the convergent evolution of aquatic adaptations in tetrapods.

Main Methods:

  • RNA sequencing (RNA-seq) was performed on eight interdigital web samples from embryonic Pelodiscus sinensis at stage TK19.
  • Differentially expressed genes (DEGs) were identified between samples.
  • Whole-mount in situ hybridization (WISH) and real-time quantitative PCR (RT-qPCR) were used to validate transcriptomic findings.

Main Results:

  • A total of 608 differentially expressed genes (DEGs) were identified in the developing interdigital webs.
  • Validation using WISH and RT-qPCR confirmed the accuracy of the RNA-seq data for selected genes.
  • The study identified potential genetic regulators of ICD and web formation.

Conclusions:

  • This study provides valuable transcriptomic data for understanding the evolution of aquatic adaptations and limb development in tetrapods.
  • The identified genes offer potential molecular targets for studying the plasticity of programmed cell death and senescence.
  • Findings contribute to comparative genomics and the study of adaptive convergent evolution.