Comprehensive transcriptome profiling in sepsis induced by pyogenic liver abscess.

Area of Science:

• Molecular biology
• Genomics
• Immunology

Background:

• Sepsis is a life-threatening condition with high mortality, often stemming from infections like pyogenic liver abscess (PLA).
• Sepsis secondary to pyogenic liver abscess (SLA) presents complex challenges in understanding its pathogenesis and effective treatment.

Purpose of the Study:

• To identify critical genes and non-coding RNAs implicated in the development and treatment of SLA.
• To elucidate the molecular mechanisms underlying SLA pathogenesis and therapeutic response.

Main Methods:

• RNA sequencing was performed on peripheral blood samples from healthy controls and SLA patients (before and after therapy).
• Integrated bioinformatics analysis was used to identify differentially expressed mRNAs, lncRNAs, circRNAs, and miRNAs.
• lncRNA/circRNA-miRNA-mRNA networks were constructed to explore regulatory relationships, with validation via RT-PCR.

Main Results:

• Thousands of differentially expressed genes and non-coding RNAs were identified between SLA patients and controls, and between pre- and post-treatment states.
• Key molecular pathways, including immune response, B cell receptor signaling, and MAPK signaling, were significantly associated with DE RNAs.
• Validated regulatory networks (ceRNAs) provide insights into the complex molecular interactions in SLA.

Conclusions:

• This study provides a comprehensive molecular landscape of SLA, highlighting key regulatory RNAs and pathways.
• The identified molecular targets and pathways offer potential for developing novel diagnostic and therapeutic strategies for SLA.
• Findings contribute to a deeper understanding of sepsis pathogenesis, particularly in the context of liver abscess infection.